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. 1990 Mar;64(3):1207–1216. doi: 10.1128/jvi.64.3.1207-1216.1990

Isolation and partial characterization of an unusual human immunodeficiency retrovirus from two persons of west-central African origin.

R De Leys 1, B Vanderborght 1, M Vanden Haesevelde 1, L Heyndrickx 1, A van Geel 1, C Wauters 1, R Bernaerts 1, E Saman 1, P Nijs 1, B Willems 1, et al.
PMCID: PMC249235  PMID: 2304140

Abstract

An unusual human retrovirus was isolated from two patients with persistent generalized lymphadenopathy who originate from West-Central Africa and are currently residing in Belgium. Although the virus shared a number of the same biological and morphological properties as human immunodeficiency retrovirus type 1 (HIV-1) and HIV-2, significant antigenic differences could be demonstrated. Several of the viral proteins also differed in molecular weight from the corresponding HIV-1 and HIV-2 proteins. Partial chemical cleavage of the most highly conserved viral proteins resulted in patterns which differed from those of HIV-1 and HIV-2. Furthermore, nucleic acid hybridization experiments were capable of discriminating between the virus types. Sequence analysis of the viral U3 region revealed a unique enhancer organization not found in other immunodeficiency viruses. The data indicated that the new isolate is more closely related to HIV-1 than to HIV-2 but clearly differs in a number of important respects.

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Selected References

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