Correction for Speiser et al., Unmodified self antigen triggers human CD8 T cells with stronger tumor reactivity than altered antigen

IMMUNOLOGY. For the article “Unmodified self antigen triggers human CD8 T cells with stronger tumor reactivity than altered antigen,” by Daniel E. Speiser, Petra Baumgaertner, Verena Voelter, Estelle Devevre, Catherine Barbey, Nathalie Rufer, and Pedro Romero, which appeared in issue 10, March 11, 2008, of Proc Natl Acad Sci USA (105:3849–3854; first published March 4, 2008; 10.1073/pnas.0800080105), the authors note that, due to a printer's error, in Fig. 1B, the x-axis label was omitted from the graph. The corrected figure and its legend appear below.

Fig. 1.

Melan-A-specific T cell responses detected ex vivo after vaccination with peptide, CpG, and IFA. PBMCs were analyzed ex vivo before vaccination and after two and four vaccinations (vacc) by flow cytometry, using CD8-specific antibodies and HLA-A2/Melan-A^MART-1 multimers (formerly called tetramers). (A) Representative data from patient LAU 972 showing low percentages of multimer^pos CD8 T cells before vaccination (Left), followed by strongly increased T cell frequencies after two (Center) and four (Right) vaccinations. (B) Corresponding data from six patients vaccinated with Melan-A natural peptide EAAGIGILTV. As an exception, patient LAU 660 received only three instead of four vaccinations. (C) Comparable data from six patients vaccinated with Melan-A analog peptide ELAGIGILTV. (D and E) Analysis with multimers constructed with natural vs. analog peptide for representative patients vaccinated with natural peptide (LAU 1013) (D Upper) and analog peptide (LAU 205) (D Lower) and for all 12 patients (E). Data in A–C were generated with analog peptide multimers. All values are percentages of multimer^pos cells of CD8^pos T cells (equal to 100%). nd, not done.