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. 1990 Mar;64(3):1360–1365. doi: 10.1128/jvi.64.3.1360-1365.1990

A 69-base-pair monkey DNA sequence enhances simian virus 40 replication and transcription through multiple motifs.

P Szymanski 1, M Woodworth 1
PMCID: PMC249257  PMID: 2154615

Abstract

We have determined that a 69-base-pair (bp) monkey DNA sequence, previously found to enhance simian virus 40 replication, has transcriptional enhancer activity as well. Consensus recognition sites for the transcription factor AP-1, present at each end of this sequence, are partially responsible for its replication- and transcription-enhancing activities. Other motifs within the 69-bp monkey sequence also act to increase the levels of replication and transcription. The activity of the monkey sequence is augmented by the presence of a simian virus 40 21-bp repeat. The 69-bp sequence enhances transcription but not replication from a distance. We conclude that the stimulation of replication and transcription can be uncoupled, suggesting that different mechanisms may be involved.

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Selected References

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