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. 1990 Apr;64(4):1698–1703. doi: 10.1128/jvi.64.4.1698-1703.1990

Immunization with baculovirus-expressed VP4 protein passively protects against simian and murine rotavirus challenge.

E R Mackow 1, P T Vo 1, R Broome 1, D Bass 1, H B Greenberg 1
PMCID: PMC249307  PMID: 2157052

Abstract

A baculovirus-expressed VP4 protein derived from the simian rhesus rotavirus (RRV) was used to parenterally immunize murine dams. VP4-immunized dams developed high levels of neutralizing antibodies against RRV and low levels of cross-reactive neutralizing antibodies against human strains Wa, ST3, and S2 and animal strains SA-11, NCDV, and Eb. Newborn mice suckled on VP4-immunized dams were protected against a virulent challenge dose of the simian strain RRV and against murine rotavirus Eb. The cross-reactive nature of the serum-neutralizing response generated by VP4 immunization and the protective efficacy of the immunization suggest that recombinant-expressed VP4 proteins should be considered as viable vaccine candidates.

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Selected References

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