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. 1990 Apr;64(4):1855–1858. doi: 10.1128/jvi.64.4.1855-1858.1990

cis-acting regulatory elements near the Epstein-Barr virus latent-infection membrane protein transcriptional start site.

D Ghosh 1, E Kieff 1
PMCID: PMC249329  PMID: 2157069

Abstract

Epstein-Barr virus (EBV) latent-infection membrane protein (LMP) gene cis-acting regulatory sequences were assayed in human B lymphocytes by using chloramphenicol acetyltransferase (cat) gene expression as a reporter. The activities of progressively longer upstream elements from bases -55 to -2350 were compared. At least two positive cis-activating regulatory components (-155 to -147 and -234 to -205) upstream of the LMP promoter were defined. LMP promoter cat gene constructs were more active in a Burkitt's lymphoma cell line latently infected with the B95 EBV strain than in the same cells latently infected with the P3HR1 EBV strain. Since the P3HR1- and B95-infected cells differ in EBNA-2 and EBNA-LP expression, EBNA-2 or EBNA-LP is a likely transactivator of the LMP promoter. Probable cognate sequences for known transcription factors in the LMP promoter are discussed.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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