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. 1990 May;64(5):2448–2451. doi: 10.1128/jvi.64.5.2448-2451.1990

Removal of cryptic poxvirus transcription termination signals from the human immunodeficiency virus type 1 envelope gene enhances expression and immunogenicity of a recombinant vaccinia virus.

P L Earl 1, A W Hügin 1, B Moss 1
PMCID: PMC249414  PMID: 2182912

Abstract

The in vivo role of the proposed poxvirus early transcription termination signal TTTTTNT was confirmed by analysis of the RNA species made by recombinant vaccinia viruses. Premature transcription termination occurred following each of two TTTTTNT sequences present naturally within the coding region of the human immunodeficiency virus type 1 envelope gene. Alteration of the TTTTTNT sequences, without changing the encoded amino acids, resulted in production of full-length early mRNAs, improved protein expression, and a more consistent immune response.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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