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. 1990 Jun;64(6):2553–2559. doi: 10.1128/jvi.64.6.2553-2559.1990

CD4-independent, productive human immunodeficiency virus type 1 infection of hepatoma cell lines in vitro.

Y Z Cao 1, A E Friedman-Kien 1, Y X Huang 1, X L Li 1, M Mirabile 1, T Moudgil 1, D Zucker-Franklin 1, D D Ho 1
PMCID: PMC249431  PMID: 2159530

Abstract

Five hepatoma cell lines, including CZHC/8571, PLC/PRF/5, Hep3B, HepG2, and HUH7, were inoculated with three diverse isolates of human immunodeficiency virus type 1 (HIV-1). Productive infection was noted in all hepatoma cell lines, and expression of viral p24 antigen lasted for over 3 months, but its level decreased in proportion to the number of viable cells. HIV-1 antigens were also found in the cells by immunohistochemical staining and radioimmunoprecipitation assay, as were viral RNA by in situ hybridization and HIV-1-like particles by electron microscopy. Virus yield assays were also positive on supernatant fluids collected from hepatoma cultures inoculated with HIV-1. Despite their susceptibility to infection, all five hepatoma cell lines were negative for CD4 by immunofluorescence and for CD4 mRNA by slot-blot hybridization. In addition, HIV-1 infection of hepatoma cell lines was not blocked by anti-CD4 monoclonal antibody or soluble CD4. Together, these findings clearly demonstrate that all five hepatoma cell lines were susceptible to productive infection by HIV-1 in vitro via a CD4-independent mechanism.

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Selected References

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