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. 1990 Jun;64(6):2577–2581. doi: 10.1128/jvi.64.6.2577-2581.1990

In vitro inhibition of hemopoietic cell line growth by hepatitis B virus.

H S Steinberg 1, P Bouffard 1, C Trépo 1, J B Zeldis 1
PMCID: PMC249434  PMID: 2335813

Abstract

The effects of hepatitis B virus (HBV) on established human cell lines of various tissue origins were evaluated by clonal or colorimetric assays in methylcellulose culture. HBV exposure inhibited the growth of six hemopoietic cell lines, while similar incubation did not affect the growth of seven nonhemopoietic carcinoma cell lines of breast, colon, liver, and stomach origin. The inhibition of hemopoietic cell line colony formation was dependent on the presence of intact viral (Dane) particles and the ratio of exposure of virions to cells and was reversible with antibodies to pre-S1, pre-S2, and S envelope protein epitopes. Purified HBV DNA, surface antigen pre-S antigens, and core antigen did not inhibit cell line growth. These results further demonstrate the tropism of HBV for cells of hemopoietic origin, confirming our previous findings on the effects of HBV on the growth of normal bone marrow progenitor cells in vitro. Established human tissue culture cell lines may be used to study the interactions of hemopoietic cells with HBV.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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