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. 1990 Jun;64(6):2819–2824. doi: 10.1128/jvi.64.6.2819-2824.1990

Hepadnavirus envelope proteins regulate covalently closed circular DNA amplification.

J Summers 1, P M Smith 1, A L Horwich 1
PMCID: PMC249463  PMID: 2335817

Abstract

Primary duck hepatocytes were infected with a mutant duck hepatitis B virus defective in envelope protein but competent for viral DNA synthesis. Cells infected by this mutant accumulated higher levels of viral covalently closed, circular DNA (cccDNA) than those infected by wild-type virus. The accumulation of high levels of cccDNA was due to a failure of the mutant-infected cells to suppress de novo cccDNA synthesis compared with suppression by cells infected by the wild type. The envelope-defective virus failed to establish a persistent infection in vitro, possibly because of a virus-mediated cell death. Therefore, one or both viral envelope proteins are required for regulation of cccDNA synthesis and for maintenance of persistent infection in vitro.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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