FIG. 7.

HMGB1 antibody (Ab) treatment regulates T-cell subpopulations. PLN and splenic T-cells originated from HMGB1 antibody–treated mice and control mice were harvested for intracellular IFN-γ staining. The percentages of CD4⁺ and CD8⁺ T-cells that are also positive for IFN-γ were determined by flow cytometric analysis. The data are presented in the table as means ± SD of three independent experiments performed. The number of CD8⁺IFN-γ⁺ T-cells (Tc1) in the PLN of antibody-treated animals was significantly higher than that of control animals (P < 0.01). A similar trend was also observed in the splenic T-cells. *P < 0.01.