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. 1990 Jun;64(6):2876–2883. doi: 10.1128/jvi.64.6.2876-2883.1990

Plasmid origin of replication of herpesvirus papio: DNA sequence and enhancer function.

D D Loeb 1, N S Sung 1, R L Pesano 1, C J Sexton 1, C Hutchison 3rd 1, J S Pagano 1
PMCID: PMC249470  PMID: 2159548

Abstract

Herpesvirus papio (HVP) is a lymphotropic virus of baboons which is related to Epstein-Barr virus (EBV) and produces latent infection. The nucleotide sequence of the 5,775-base-pair (bp) EcoRI K fragment of HVP, which has previously been shown to confer the ability to replicate autonomously, has been determined. Within this DNA fragment is a region which bears structural and sequence similarity to the ori-P region of EBV. The HVP ori-P region has a 10- by 26-bp tandem array which is related to the 20- by 30-bp tandem array from the EBV ori-P region. In HVP there is an intervening region of 764 bp followed by five partial copies of the 26-bp monomer. Both the EBV and HVP 3' regions have the potential to form dyad structures which, however, differ in arrangement. We also demonstrate that a transcriptional enhancer which requires transactivation by a virus-encoded factor is present in the HVP ori-P.

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Selected References

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