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. 1990 Jun;64(6):3122–3125. doi: 10.1128/jvi.64.6.3122-3125.1990

Processing of pseudorabies virus glycoprotein gII.

U Wölfer 1, V Kruft 1, D Sawitzky 1, H Hampl 1, B Wittmann-Liebold 1, K O Habermehl 1
PMCID: PMC249507  PMID: 2159568

Abstract

The glycoprotein complex gII of pseudorabies virus was isolated by immunoprecipitation with the monoclonal antibody M5, which was covalently linked to protein A-Sepharose. After sodium dodecyl sulfate-polyarylamide gel electrophoresis under reducing conditions and blotting onto poly(vinylidene difluoride) membrane, its subunits, gIIa, gIIb, and gIIc, were subjected to N-terminal sequencing. gIIa and gIIb start at position 59 and gIIc starts at position 503 according to the amino acid sequence deduced from the gene, indicating that there is one major protein (gIIa) which is cleaved into the two protein fragments gIIb and gIIc. Protein labeling with 14C-amino acids gave no indication that the three proteins (gIIa, gIIb, and gIIc) of the complex are present in equimolar ratios. It seems that gIIa is only a minor component of the complex, whereas gIIb and gIIc are contained in equimolar amounts.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Hampl H., Ben-Porat T., Ehrlicher L., Habermehl K. O., Kaplan A. S. Characterization of the envelope proteins of pseudorabies virus. J Virol. 1984 Nov;52(2):583–590. doi: 10.1128/jvi.52.2.583-590.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Hughes J. H., Mack K., Hamparian V. V. India ink staining of proteins on nylon and hydrophobic membranes. Anal Biochem. 1988 Aug 15;173(1):18–25. doi: 10.1016/0003-2697(88)90152-2. [DOI] [PubMed] [Google Scholar]
  3. KAPLAN A. S., VATTER A. E. A comparison of herpes simplex and pseudorabies viruses. Virology. 1959 Apr;7(4):394–407. doi: 10.1016/0042-6822(59)90068-6. [DOI] [PubMed] [Google Scholar]
  4. Keller P. M., Davison A. J., Lowe R. S., Bennett C. D., Ellis R. W. Identification and structure of the gene encoding gpII, a major glycoprotein of varicella-zoster virus. Virology. 1986 Jul 15;152(1):181–191. doi: 10.1016/0042-6822(86)90383-1. [DOI] [PubMed] [Google Scholar]
  5. Laemmli U. K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970 Aug 15;227(5259):680–685. doi: 10.1038/227680a0. [DOI] [PubMed] [Google Scholar]
  6. Lukàcs N., Thiel H. J., Mettenleiter T. C., Rziha H. J. Demonstration of three major species of pseudorabies virus glycoproteins and identification of a disulfide-linked glycoprotein complex. J Virol. 1985 Jan;53(1):166–173. doi: 10.1128/jvi.53.1.166-173.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Mettenleiter T. C., Lukàcs N., Thiel H. J., Schreurs C., Rziha H. J. Location of the structural gene of pseudorabies virus glycoprotein complex gII. Virology. 1986 Jul 15;152(1):66–75. doi: 10.1016/0042-6822(86)90372-7. [DOI] [PubMed] [Google Scholar]
  8. Robbins A. K., Dorney D. J., Wathen M. W., Whealy M. E., Gold C., Watson R. J., Holland L. E., Weed S. D., Levine M., Glorioso J. C. The pseudorabies virus gII gene is closely related to the gB glycoprotein gene of herpes simplex virus. J Virol. 1987 Sep;61(9):2691–2701. doi: 10.1128/jvi.61.9.2691-2701.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Ross L. J., Sanderson M., Scott S. D., Binns M. M., Doel T., Milne B. Nucleotide sequence and characterization of the Marek's disease virus homologue of glycoprotein B of herpes simplex virus. J Gen Virol. 1989 Jul;70(Pt 7):1789–1804. doi: 10.1099/0022-1317-70-7-1789. [DOI] [PubMed] [Google Scholar]
  10. Schneider C., Newman R. A., Sutherland D. R., Asser U., Greaves M. F. A one-step purification of membrane proteins using a high efficiency immunomatrix. J Biol Chem. 1982 Sep 25;257(18):10766–10769. [PubMed] [Google Scholar]
  11. Spaete R. R., Thayer R. M., Probert W. S., Masiarz F. R., Chamberlain S. H., Rasmussen L., Merigan T. C., Pachl C. Human cytomegalovirus strain Towne glycoprotein B is processed by proteolytic cleavage. Virology. 1988 Nov;167(1):207–225. doi: 10.1016/0042-6822(88)90071-2. [DOI] [PubMed] [Google Scholar]
  12. Walsh M. J., McDougall J., Wittmann-Liebold B. Extended N-terminal sequencing of proteins of archaebacterial ribosomes blotted from two-dimensional gels onto glass fiber and poly(vinylidene difluoride) membrane. Biochemistry. 1988 Sep 6;27(18):6867–6876. doi: 10.1021/bi00418a032. [DOI] [PubMed] [Google Scholar]
  13. Whalley J. M., Robertson G. R., Scott N. A., Hudson G. C., Bell C. W., Woodworth L. M. Identification and nucleotide sequence of a gene in equine herpesvirus 1 analogous to the herpes simplex virus gene encoding the major envelope glycoprotein gB. J Gen Virol. 1989 Feb;70(Pt 2):383–394. doi: 10.1099/0022-1317-70-2-383. [DOI] [PubMed] [Google Scholar]

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