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. 1990 Jun;64(6):3135–3138. doi: 10.1128/jvi.64.6.3135-3138.1990

Rapid titration of retroviral vectors encoding intracellular antigens by flow cytometry.

T L Sladek 1, J W Jacobberger 1
PMCID: PMC249510  PMID: 2159569

Abstract

Flow cytometry was used to detect cells infected with retroviral vectors encoding both simian virus 40 large T antigen and G418 resistance after indirect immunofluorescence staining using a T-antigen-specific monoclonal antibody and a fluorescein-conjugated secondary antibody. Titers of viral stocks determined by flow cytometry were equivalent to those determined by quantitation of G418-resistant colonies.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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