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. 1969 Jan;97(1):6–12. doi: 10.1128/jb.97.1.6-12.1969

Genetic Relationship Between Sarcina ureae and Members of the Genus Bacillus

Saundra E Herndon a,1, Kenneth F Bott a
PMCID: PMC249532  PMID: 5764347

Abstract

Deoxyribonucleic acid-ribonucleic acid (DNA-RNA) and DNA-DNA hybridization studies were performed to determine the degree of genetic relatedness between Sarcina ureae and several members of the genus Bacillus. DNA-RNA hybridization showed a high degree of homology between S. ureae RNA and DNA from Bacillus species having a similar guanine plus cytosine content. The DNA from other genera of the family Micrococcaceae showed less homology with S. ureae RNA than did that of the Bacillus species tested; however, this homology was not found between the DNA of S. ureae and DNA from these Bacillus species or DNA from the other Micrococcaceae tested. Transformation with Bacillus DNA, infection with representatives from several major classes of Bacillus phages, and electrophoretic analysis of proteins in crude extracts of these strains were also attempted as a further test of the genetic relationship between the genera. These experiments did not support the belief that the two groups are closely related genetically.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Auletta A. E., Kennedy E. R. Deoxyribonucleic acid base composition of some members of the Micrococcaceae. J Bacteriol. 1966 Jul;92(1):28–34. doi: 10.1002/path.1700920103. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. BOLTON E. T., McCARTHY B. J. A general method for the isolation of RNA complementary to DNA. Proc Natl Acad Sci U S A. 1962 Aug;48:1390–1397. doi: 10.1073/pnas.48.8.1390. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Bott K. F., Wilson G. A. Development of competence in the Bacillus subtilis transformation system. J Bacteriol. 1967 Sep;94(3):562–570. doi: 10.1128/jb.94.3.562-570.1967. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. DOI R. H., IGARASHI R. T. CONSERVATION OF RIBOSOMAL AND MESSENGER RIBONUCLEIC ACID CISTRONS IN BACILLUS SPECIES. J Bacteriol. 1965 Aug;90:384–390. doi: 10.1128/jb.90.2.384-390.1965. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Doi R. H., Igarashi R. T. Heterogeneity of the conserved ribosomal ribonucleic acid sequences of Bacillus subtilis. J Bacteriol. 1966 Jul;92(1):88–96. doi: 10.1128/jb.92.1.88-96.1966. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Dubnau D., Smith I., Morell P., Marmur J. Gene conservation in Bacillus species. I. Conserved genetic and nucleic acid base sequence homologies. Proc Natl Acad Sci U S A. 1965 Aug;54(2):491–498. doi: 10.1073/pnas.54.2.491. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Gillespie D., Spiegelman S. A quantitative assay for DNA-RNA hybrids with DNA immobilized on a membrane. J Mol Biol. 1965 Jul;12(3):829–842. doi: 10.1016/s0022-2836(65)80331-x. [DOI] [PubMed] [Google Scholar]
  8. Heberlein G. T., De Ley J., Tijtgat R. Deoxyribonucleic acid homology and taxonomy of Agrobacterium, Rhizobium, and Chromobacterium. J Bacteriol. 1967 Jul;94(1):116–124. doi: 10.1128/jb.94.1.116-124.1967. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Hill L. R. An index to deoxyribonucleic acid base compositions of bacterial species. J Gen Microbiol. 1966 Sep;44(3):419–437. doi: 10.1099/00221287-44-3-419. [DOI] [PubMed] [Google Scholar]
  10. IANDOLO J. J., ORDAL Z. J. GERMINATION SYSTEM FOR ENDOSPORES OF SARCINA UREAE. J Bacteriol. 1964 Jan;87:235–236. doi: 10.1128/jb.87.1.235-236.1964. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Legault-Démare J., Desseaux B., Heyman T., Séror S., Ress G. P. Studies on hybrid molecules of nucleic acids. I. DNA-DNA hybrids on nitrocellulose filters. Biochem Biophys Res Commun. 1967 Aug 23;28(4):550–557. doi: 10.1016/0006-291x(67)90349-x. [DOI] [PubMed] [Google Scholar]
  12. MARMUR J., FALKOW S., MANDEL M. NEW APPROACHES TO BACTERIAL TAXONOMY. Annu Rev Microbiol. 1963;17:329–372. doi: 10.1146/annurev.mi.17.100163.001553. [DOI] [PubMed] [Google Scholar]
  13. MASTER R. W. POSSIBLE SYNTHESIS OF POLYRIBONUCLEOTIDES OF KNOWN BASE-TRIPLET SEQUENCES. Nature. 1965 Apr 3;206:93–93. doi: 10.1038/206093b0. [DOI] [PubMed] [Google Scholar]
  14. MCCARTHY B. J., BOLTON E. T. An approach to the measurement of genetic relatedness among organisms. Proc Natl Acad Sci U S A. 1963 Jul;50:156–164. doi: 10.1073/pnas.50.1.156. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Mazanec K., Kocur M., Martinec T. Electron Microscopy of Ultrathin Sections of Sporosarcina ureae. J Bacteriol. 1965 Sep;90(3):808–816. doi: 10.1128/jb.90.3.808-816.1965. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. SAITO H., MIURA K. I. PREPARATION OF TRANSFORMING DEOXYRIBONUCLEIC ACID BY PHENOL TREATMENT. Biochim Biophys Acta. 1963 Aug 20;72:619–629. [PubMed] [Google Scholar]
  17. Spizizen J. TRANSFORMATION OF BIOCHEMICALLY DEFICIENT STRAINS OF BACILLUS SUBTILIS BY DEOXYRIBONUCLEATE. Proc Natl Acad Sci U S A. 1958 Oct 15;44(10):1072–1078. doi: 10.1073/pnas.44.10.1072. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. THOMPSON R. S., LEADBETTER E. R. On the isolation of dipicolinic acid fromhendospores of Sarcina ureae. Arch Mikrobiol. 1963;45:27–32. doi: 10.1007/BF00410294. [DOI] [PubMed] [Google Scholar]
  19. Venner H. Taxonomy of Sarcina on the basis of their DNA base composition. Acta Biochim Pol. 1967;14(1):31–40. [PubMed] [Google Scholar]
  20. Wilson G. A., Bott K. F. Nutritional factors influencing the development of competence in the Bacillus subtilis transformation system. J Bacteriol. 1968 Apr;95(4):1439–1449. doi: 10.1128/jb.95.4.1439-1449.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]

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