Abstract
Subunit influenza vaccines have the advantage of purity and a broad dosage range. Clinical studies were done with a new subunit vaccine produced by detergent fractionation with tri-(n-butyl) phosphate (TNBP). Acceptability by the recipients was compared in volunteers given 400 CCA units of A2 and 300 of B Influenza vaccine as either Sharples, zonal centrifuged, ether subunit, or TNBP subunit bivalent vaccines. When given subcutaneously, subunit vaccine caused only slightly less pain and erythema induration than zonal or Sharples vaccine. Compared with the i.m. route, the same vaccine given subcutaneously produced twice the local pain and eight times the erythema induration. Fever was infrequent and low grade by either route. HI antibody rises were good in all groups. Increasing dosages of TNBP vaccine up to 6400 CCA units of A2 and 4800 of B given i.m. as monovalent vaccine, produced no local and few febrile responses. An augmentation of the antibody titre occurred with the highest CCA unit dosages. Subunit vaccine given i.m. permitted administration of high CCA unit dosage beyond that accepted for standard vaccine without any increase in local reactions and with a measurable increase in antibody production and the induction of antibodies against related heterologous strains.
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Selected References
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- Fazekas de Saint Groth The antigenic subunits of influenza viruses. II. The spectrum of cross reactions. J Immunol. 1969 Nov;103(5):1107–1115. [PubMed] [Google Scholar]
- Fulk R. V., Fedson D. S., Huber M. A., Fitzpatrick J. R., Howar B. F., Kasel J. A. Antibody responses in children and elderly persons following local or parenteral administration of an inactivated influenza virus vaccine, A2-Hong Kong-68 variant. J Immunol. 1969 Apr;102(4):1102–1105. [PubMed] [Google Scholar]
- Mostow S. R., Schoenbaum S. C., Dowdle W. R., Coleman M. T., Kaye H. S., Hierholzer J. C. Studies on inactivated influenza vaccines. II. Effect of increasing dosage on antibody response and adverse reactions in man. Am J Epidemiol. 1970 Oct;92(4):248–256. doi: 10.1093/oxfordjournals.aje.a121204. [DOI] [PubMed] [Google Scholar]
- Mostow S. R., Schoenbaum S. C., Dowdle W. R., Coleman M. T., Kaye H. S. Studies with inactivated influenza vaccines purified by zonal centrifugation. 1. Adverse reactions and serological responses. Bull World Health Organ. 1969;41(3):525–530. [PMC free article] [PubMed] [Google Scholar]
- Neurath A. R., Rubin B. A., Sillaman J., Tint H. The effect of nonaqueous solvents on the quaternary structure of viruses: a procedure for the simultaneous concentration, purification and disruption of influenza viruses. Microbios. 1971 Sep;4(14):145–150. [PubMed] [Google Scholar]
- Phillips C. A., Forsyth B. R., Christmas W. A., Gump D. W., Whorton E. B., Rogers I., Rudin A. Purified influenza vaccine: clinical and serologic responses to varying doses and different routes of immunization. J Infect Dis. 1970 Jul-Aug;122(1):26–32. doi: 10.1093/infdis/122.1-2.26. [DOI] [PubMed] [Google Scholar]
- Reimer C. B., Baker R. S., Van Frank R. M., Newlin T. E., Cline G. B., Anderson N. G. Purification of large quantities of influenza virus by density gradient centrifugation. J Virol. 1967 Dec;1(6):1207–1216. doi: 10.1128/jvi.1.6.1207-1216.1967. [DOI] [PMC free article] [PubMed] [Google Scholar]
- SEVER J. L. Application of a microtechnique to viral serological investigations. J Immunol. 1962 Mar;88:320–329. [PubMed] [Google Scholar]
- Tauraso N. M., Gleckman R., Pedreira F. A., Sabbaj J., Yahwak R., Madoff M. A. Effect of dosage and route of inoculation upon antigenicity of inactivated influenza virus vaccine (Hong Kong strain) in man. Bull World Health Organ. 1969;41(3):507–516. [PMC free article] [PubMed] [Google Scholar]