Abstract
The growth of Streptococcus faecalis is inhibited by propionate, and the inhibition is reversed by lipoic acid or acetate. A study of the role of pyruvate oxidation in S. faecalis showed that propionate inhibited the lipoic acid-dependent aerobic oxidation of pyruvate in resting cells. Pyruvate dehydrogenation with neotetrazolium as a hydrogen acceptor in cell-free extracts also required lipoic acid and was markedly inhibited by propionyl phosphate as well as sodium propionate. Some lipid substances, such as palmitate, oleate, behenate, and lecithin, had a lipoic acid-replacing effect on growth of the organism. Biotin or bicarbonate promoted the lipoic acid-dependent growth. Acetate-2-14C added to the medium was mainly incorporated into the lipid fraction of the cells. Evolution of 14CO2 from pyruvate-2-14C was not observed in resting cells of the organism, even under aerobic conditions. From the above findings, it is concluded that lipid synthesis through pyruvate oxidation plays a very important role in bacterial growth in medium containing propionate.
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- DAVIS R. J., HUTNER S. H., SEAMAN G. R., STOKSTAD E. L. Assay of thioctic acid. Methods Biochem Anal. 1956;3:23–47. doi: 10.1002/9780470110195.ch2. [DOI] [PubMed] [Google Scholar]
- DEIBEL R. H. THE GROUP D STREPTOCOCCI. Bacteriol Rev. 1964 Sep;28:330–366. doi: 10.1128/br.28.3.330-366.1964. [DOI] [PMC free article] [PubMed] [Google Scholar]
- HILL C. H. Studies on the inhibition of growth of Streptococcus faecalis by sodium propionate. J Biol Chem. 1952 Nov;199(1):329–332. [PubMed] [Google Scholar]
- LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
- O'kane D. J., Gunsalus I. C. Pyruvic Acid Metabolism: A Factor Required for Oxidation by Streptococcus faecalis. J Bacteriol. 1948 Oct;56(4):499–506. doi: 10.1128/jb.56.4.499-506.1948. [DOI] [PMC free article] [PubMed] [Google Scholar]
- REED L. J., LEACH F. R., KOIKE M. Studies on a lipoic acid-activating system. J Biol Chem. 1958 May;232(1):123–142. [PubMed] [Google Scholar]
- ROSE I. A., GRUNBERG-MANAGO M., KOREY S. R., OCHOA S. Enzymatic phosphorylation of acetate. J Biol Chem. 1954 Dec;211(2):737–756. [PubMed] [Google Scholar]