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. 1969 Jan;97(1):210–216. doi: 10.1128/jb.97.1.210-216.1969

Transductional Analysis of Pseudomonas aeruginosa Methionineless Auxotrophs

D H Calhoun 1, T W Feary 1
PMCID: PMC249579  PMID: 4974390

Abstract

Seventy-one methionineless and cysteineless auxotrophs of Pseudomonas aeruginosa were placed into nine groups on the basis of their growth on methionine precursors and the cross-feeding response. Transduction experiments with bacteriophage F116 indicated the presence of four linkage groups among the methionineless mutants and at least three among the cysteineless mutants. These studies suggested that the biosynthesis of methionine in P. aeruginosa is similar to that described in other microorganisms, although none of the mutants lacking the ability to methylate homocysteine grew with vitamin B12 or S-adenosylmethionine.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. CLOWES R. C. Investigation of the genetics of cysteineless mutants of Salmonella typhimurium by transduction. J Gen Microbiol. 1958 Feb;18(1):154–172. doi: 10.1099/00221287-18-1-154. [DOI] [PubMed] [Google Scholar]
  2. Crawford I. P., Gunsalus I. C. Inducibility of tryptophan synthetase in Pseudomonas putida. Proc Natl Acad Sci U S A. 1966 Aug;56(2):717–724. doi: 10.1073/pnas.56.2.717. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. DAVIS B. D., MINGIOLI E. S. Mutants of Escherichia coli requiring methionine or vitamin B12. J Bacteriol. 1950 Jul;60(1):17–28. doi: 10.1128/jb.60.1.17-28.1950. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. GIBSON F., WOODS D. D. The synthesis of methionine by suspensions of Escherichia coli. Biochem J. 1960 Jan;74:160–172. doi: 10.1042/bj0740160. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Grabow W. O., Smit J. A. Methionine synthesis in Proteus mirabilis. J Gen Microbiol. 1967 Jan;46(1):47–57. doi: 10.1099/00221287-46-1-47. [DOI] [PubMed] [Google Scholar]
  6. HOLLOWAY B. W., EGAN J. B., MONK M. Lysogeny in Pseudomonas aeruginosa. Aust J Exp Biol Med Sci. 1960 Aug;38:321–329. doi: 10.1038/icb.1960.34. [DOI] [PubMed] [Google Scholar]
  7. HOLLOWAY B. W. Genetic recombination in Pseudomonas aeruginosa. J Gen Microbiol. 1955 Dec;13(3):572–581. doi: 10.1099/00221287-13-3-572. [DOI] [PubMed] [Google Scholar]
  8. JACOB F., MONOD J. Genetic regulatory mechanisms in the synthesis of proteins. J Mol Biol. 1961 Jun;3:318–356. doi: 10.1016/s0022-2836(61)80072-7. [DOI] [PubMed] [Google Scholar]
  9. Lawrence D. A., Smith D. A., Rowbury R. J. Regulation of methionine synthesis in Salmonella typhimurium: mutants resistant to inhibition by analogues of methionine. Genetics. 1968 Apr;58(4):473–492. doi: 10.1093/genetics/58.4.473. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Mee B. J., Lee B. T. An analysis of histidine requiring mutants in Pseudomonas aeruginosa. Genetics. 1967 Apr;55(4):709–722. doi: 10.1093/genetics/55.4.709. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Ornston L. N. The conversion of catechol and protocatechuate to beta-ketoadipate by Pseudomonas putida. IV. Regulation. J Biol Chem. 1966 Aug 25;241(16):3800–3810. [PubMed] [Google Scholar]
  12. PIGG C. J., SPENCE K. D., PARKS L. W. Methionine biosynthesis in yeast. Arch Biochem Biophys. 1962 Jun;97:491–496. doi: 10.1016/0003-9861(62)90112-1. [DOI] [PubMed] [Google Scholar]
  13. Rowbury R. J., Woods D. D. The regulation of cystathionine formation in Escherichia coli. J Gen Microbiol. 1966 Jan;42(1):155–163. doi: 10.1099/00221287-42-1-155. [DOI] [PubMed] [Google Scholar]
  14. SANDERSON K. E., DEMEREC M. THE LINKAGE MAP OF SALMONELLA TYPHIMURIUM. Genetics. 1965 Jun;51:897–913. doi: 10.1093/genetics/51.6.897. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. SHAPIRO S. K., LOHMAR P., HERTENSTEIN M. Utilization of S-adenosylmethionine for the biosynthesis of methionine. Arch Biochem Biophys. 1963 Jan;100:74–79. doi: 10.1016/0003-9861(63)90036-5. [DOI] [PubMed] [Google Scholar]
  16. Smith D. A., Childs J. D. Methionine genes and enzymes of Salmonella typhimurium. Heredity (Edinb) 1966 May;21(2):265–286. doi: 10.1038/hdy.1966.22. [DOI] [PubMed] [Google Scholar]
  17. Stanier R. Y. Simultaneous Adaptation: A New Technique for the Study of Metabolic Pathways. J Bacteriol. 1947 Sep;54(3):339–348. doi: 10.1128/jb.54.3.339-348.1947. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. TAYLOR A. L., THOMAN M. S. THE GENETIC MAP OF ESCHERICHIA COLI K-12. Genetics. 1964 Oct;50:659–677. doi: 10.1093/genetics/50.4.659. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. WADE E. H., MATHESON A. T., HANES C. S. Quantitative chromatographic methods. 3. Factors controlling the patterns of separation of the amino acids on paper chromatograms. Can J Biochem Physiol. 1961 Jan;39:141–161. doi: 10.1139/o61-014. [DOI] [PubMed] [Google Scholar]
  20. WIEBERS J. L., GARNER H. R. USE OF S-METHYLCYSTINE AND CYSTATHIONINE BY METHIONINELESS NEUROSPORA MUTANTS. J Bacteriol. 1964 Dec;88:1798–1804. doi: 10.1128/jb.88.6.1798-1804.1964. [DOI] [PMC free article] [PubMed] [Google Scholar]

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