Skip to main content
NCBI home page
Journal of Bacteriology logoLink to Journal of Bacteriology
. 1969 Jan;97(1):217–222. doi: 10.1128/jb.97.1.217-222.1969

Effect of Galactose on β-Galactosidase Synthesis in Escherichia coli K-121

Barbara Llanes a,2, Elizabeth McFall a
PMCID: PMC249581  PMID: 4884812

Abstract

In wild-type strains of Escherichia coli K-12, the rate of thiomethylgalactoside (TMG)-induced β-galactosidase synthesis is decreased in the presence of galactose or glucose. A spontaneous mutant of a K-12 strain, 58-161, which synthesizes β-galactosidase at a low rate was isolated. In this mutant, galactose, after a lag of about one generation time, evoked the same final differential rate of enzyme synthesis as did the gratuitous inducer TMG. However, constitutive, TMG-induced and galactose-induced synthesis in the mutant were subject to inhibition by exogenous glucose. It is concluded that repression of β-galactosidase synthesis derived from glucose is distinct from the inhibition derived from galactose.

Full text

PDF
217

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. BURSTEIN C., COHN M., KEPES A., MONOD J. R OLE DU LACTOSE ET DE SES PRODUITS M'ETABOLIQUES DANS L'INDUCTION DE L'OP'ERON LACTOSE CHEZ ESCHERICHIA COLI. Biochim Biophys Acta. 1965 Apr 19;95:634–639. [PubMed] [Google Scholar]
  2. Beggs W. H., Rogers P. Galactose repression of beta-galactosidase induction in Escherichia coli. J Bacteriol. 1966 May;91(5):1869–1874. doi: 10.1128/jb.91.5.1869-1874.1966. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. COHEN-BAZIRE G., JOLIT M. Isolement par sélection de mutants d'Escherichia coli synthétisant spontanément l'amylomaltase et la beta-galactosidase. Ann Inst Pasteur (Paris) 1953 May;84(5):937–945. [PubMed] [Google Scholar]
  4. COOK A., LEDERBERG J. Recombination studies of lactose nonfermenting mutants of Escherichia coli K-12. Genetics. 1962 Oct;47:1335–1353. doi: 10.1093/genetics/47.10.1335. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Demerec M., Adelberg E. A., Clark A. J., Hartman P. E. A proposal for a uniform nomenclature in bacterial genetics. Genetics. 1966 Jul;54(1):61–76. doi: 10.1093/genetics/54.1.61. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Ganesan A. K., Rotman B. Transport systems for galactose and galactosides in Escherichia coli. I. Genetic determination and regulation of the methyl-galactoside permease. J Mol Biol. 1966 Mar;16(1):42–50. doi: 10.1016/s0022-2836(66)80261-9. [DOI] [PubMed] [Google Scholar]
  7. JACOB F., MONOD J. Genetic regulatory mechanisms in the synthesis of proteins. J Mol Biol. 1961 Jun;3:318–356. doi: 10.1016/s0022-2836(61)80072-7. [DOI] [PubMed] [Google Scholar]
  8. KOCH A. L. THE ROLE OF PERMEASE IN TRANSPORT. Biochim Biophys Acta. 1964 Jan 27;79:177–200. doi: 10.1016/0926-6577(64)90050-6. [DOI] [PubMed] [Google Scholar]
  9. LEDERBERG J. The beta-d-galactosidase of Escherichia coli, strain K-12. J Bacteriol. 1950 Oct;60(4):381–392. doi: 10.1128/jb.60.4.381-392.1950. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. LURIA S. E., ADAMS J. N., TING R. C. Transduction of lactose-utilizing ability among strains of E. coli and S. dysenteriae and the properties of the transducing phage particles. Virology. 1960 Nov;12:348–390. doi: 10.1016/0042-6822(60)90161-6. [DOI] [PubMed] [Google Scholar]
  11. Llanes B., McFall E. Role of lac genes in induction of beta-galactosidase synthesis by galactose. J Bacteriol. 1969 Jan;97(1):223–229. doi: 10.1128/jb.97.1.223-229.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Loomis W. F., Jr, Magasanik B. Glucose-lactose diauxie in Escherichia coli. J Bacteriol. 1967 Apr;93(4):1397–1401. doi: 10.1128/jb.93.4.1397-1401.1967. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. MAGASANIK B. Catabolite repression. Cold Spring Harb Symp Quant Biol. 1961;26:249–256. doi: 10.1101/sqb.1961.026.01.031. [DOI] [PubMed] [Google Scholar]
  14. MANDELSTAM J. The repression of constitutive beta-galactosidase in Escherichia coli by glucose and other carbon sources. Biochem J. 1962 Mar;82:489–493. doi: 10.1042/bj0820489. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. MCFALL E. GENETIC STRUCTURE OF THE D-SERINE DEAMINASE SYSTEM OF ESCHERICHIA COLI. J Mol Biol. 1964 Sep;9:746–753. doi: 10.1016/s0022-2836(64)80179-0. [DOI] [PubMed] [Google Scholar]
  16. MCFALL E., MANDELSTAM J. SPECIFIC METABOLIC REPRESSION OF THREE INDUCED ENZYMES IN ESCHERICHIA COLI. Biochem J. 1963 Nov;89:391–398. doi: 10.1042/bj0890391. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Moses V., Prevost C. Catabolite repression of beta-galactosidase synthesis in Escherichia coli. Biochem J. 1966 Aug;100(2):336–353. doi: 10.1042/bj1000336. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. NAKADA D., MAGASANIK B. THE ROLES OF INDUCER AND CATABOLITE REPRESSOR IN THE SYNTHESIS OF BETA-GALACTOSIDASE BY ESCHERICHIA COLI. J Mol Biol. 1964 Jan;8:105–127. doi: 10.1016/s0022-2836(64)80153-4. [DOI] [PubMed] [Google Scholar]
  19. Paigen K. Phenomenon of transient repression in Escherichia coli. J Bacteriol. 1966 Mar;91(3):1201–1209. doi: 10.1128/jb.91.3.1201-1209.1966. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Paigen K. Role of the galactose pathway in the regulation of beta-galactosidase. J Bacteriol. 1966 Nov;92(5):1394–1403. doi: 10.1128/jb.92.5.1394-1403.1966. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. ROTMAN B. Regulation of enzymatic activity in the intact cell: the beta-D-galactosidase of Escherichia coli. J Bacteriol. 1958 Jul;76(1):1–14. doi: 10.1128/jb.76.1.1-14.1958. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Journal of Bacteriology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES