Skip to main content
NCBI home page
Journal of Bacteriology logoLink to Journal of Bacteriology
. 1969 Jan;97(1):250–255. doi: 10.1128/jb.97.1.250-255.1969

Genetic Analysis of Carbohydrate Transport-deficient Mutants of Salmonella typhimurium

Mark Levinthal a, Robert D Simoni a,1
PMCID: PMC249589  PMID: 4884816

Abstract

Mutants (car) isolated from Salmonella typhimurium were unable to utilize or ferment the following carbohydrates (all d-configuration): glucose, fructose, mannose, N-acetylglucosamine, sorbitol, mannitol, maltose, melibiose, and glycerol. The mutants did utilize galactose, glucose 6-phosphate, gluconic acid, glucuronic acid, pyruvate, and l-lactate. Biochemical analysis showed that there were two classes of mutants, each lacking one component of a phosphotransferase system. CarA mutants were deficient in enzyme I; carB lacked the phosphate carrier protein, HPr. Mapping experiments showed that the carA gene was located near pro; the carB gene mapped near purC.

Full text

PDF
250

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. EGAN J. B., MORSE M. L. CARBOHYDRATE TRANSPORT IN STAPHYLOCOCCUS AUREUS I. GENETIC AND BIOCHEMICAL ANALYSIS OF A PLEIOTROPIC TRANSPORT MUTANT. Biochim Biophys Acta. 1965 Feb 15;97:310–319. doi: 10.1016/0304-4165(65)90096-6. [DOI] [PubMed] [Google Scholar]
  2. Egan J. B., Morse M. L. Carbohydrate transport in Staphylococcus aureus. 3. Studies of the transport process. Biochim Biophys Acta. 1966 Jan 4;112(1):63–73. doi: 10.1016/s0926-6585(96)90009-6. [DOI] [PubMed] [Google Scholar]
  3. Egan J. B., Morse M. L. Carbohydrate transport in Staphylococcus aureus. II. Characterization of the defect of a pleiotropic transport mutant. Biochim Biophys Acta. 1965 Sep 27;109(1):172–183. doi: 10.1016/0926-6585(65)90101-9. [DOI] [PubMed] [Google Scholar]
  4. Fink G. R., Roth J. R. Histidine regulatory mutants in Salmonella typhiumium. VI. Dominance studies. J Mol Biol. 1968 May 14;33(3):547–557. doi: 10.1016/0022-2836(68)90305-7. [DOI] [PubMed] [Google Scholar]
  5. KUNDIG W., GHOSH S., ROSEMAN S. PHOSPHATE BOUND TO HISTIDINE IN A PROTEIN AS AN INTERMEDIATE IN A NOVEL PHOSPHO-TRANSFERASE SYSTEM. Proc Natl Acad Sci U S A. 1964 Oct;52:1067–1074. doi: 10.1073/pnas.52.4.1067. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Roth J. R., Hartman P. E. Heterogeneity in P22 transducing particles. Virology. 1965 Nov;27(3):297–307. doi: 10.1016/0042-6822(65)90109-1. [DOI] [PubMed] [Google Scholar]
  7. SANDERSON K. E., DEMEREC M. THE LINKAGE MAP OF SALMONELLA TYPHIMURIUM. Genetics. 1965 Jun;51:897–913. doi: 10.1093/genetics/51.6.897. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Sanderson K. E. Revised linkage map of Salmonella typhimurium. Bacteriol Rev. 1967 Dec;31(4):354–372. doi: 10.1128/br.31.4.354-372.1967. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Simoni R. D., Levinthal M., Kundig F. D., Kundig W., Anderson B., Hartman P. E., Roseman S. Genetic evidence for the role of a bacterial phosphotransferase system in sugar transport. Proc Natl Acad Sci U S A. 1967 Nov;58(5):1963–1970. doi: 10.1073/pnas.58.5.1963. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Simoni R. D., Smith M. F., Roseman S. Resolution of a staphylococcal phosphotransferase system into four protein components and its relation to sugar transport. Biochem Biophys Res Commun. 1968 Jun 10;31(5):804–811. doi: 10.1016/0006-291x(68)90634-7. [DOI] [PubMed] [Google Scholar]
  11. Smith H. O., Levine M. A phage P22 gene controlling integration of prophage. Virology. 1967 Feb;31(2):207–216. doi: 10.1016/0042-6822(67)90164-x. [DOI] [PubMed] [Google Scholar]
  12. Tanaka S., Fraenkel D. G., Lin E. C. The enzymatic lesion of strain MM-6, a pleiotropic carbohydrate-negative mutant of Escherichia coli. Biochem Biophys Res Commun. 1967 Apr 7;27(1):63–67. doi: 10.1016/s0006-291x(67)80040-8. [DOI] [PubMed] [Google Scholar]
  13. Tanaka S., Lin E. C. Two classes of pleiotropic mutants of Aerobacter aerogenes lacking components of a phosphoenolpyruvate-dependent phosphotransferase system. Proc Natl Acad Sci U S A. 1967 Apr;57(4):913–919. doi: 10.1073/pnas.57.4.913. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Wang R. J., Morse M. L. Carbohydrate accumulation and metabolism in Escherichia coli. I. Description of pleiotropic mutants. J Mol Biol. 1968 Feb 28;32(1):59–66. doi: 10.1016/0022-2836(68)90145-9. [DOI] [PubMed] [Google Scholar]

Articles from Journal of Bacteriology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES