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. 1969 Jan;97(1):322–327. doi: 10.1128/jb.97.1.322-327.1969

β-Cyanoalanine Formation by Chromobacterium violaceum

Miriam M Brysk 1,2,1, W A Corpe 1,2, L V Hankes 1,2
PMCID: PMC249603  PMID: 5764335

Abstract

Nonproliferating cells of Chromobacterium violaceum incubated with glycine, methionine, and succinate as substrates accumulated β-cyanoalanine in the culture fluid. Tracer experiments showed that carbons-2, -3, and -4 of β-cyanoalanine are derived from the 2-carbon of glycine. When methionine-methyl-14C, succinate-1,4-14C, or succinate-2,3-14C was used as substrate, β-cyanoalanine did not become labeled. If K14CN and serine were used as substrates, the cyano group of β-cyanoalanine was labeled. Radioactive β-cyanoalanine, labeled in the 3-carbon, was formed when glycine and H14CHO were used as substrates. 14C-formic acid did not replace formaldehyde. Asparagine also accumulated in the incubated mixture and was found to be labeled in the amide carbon. Incubation of cells with β-cyanoalanine-4-14C produced labeled aspartic acid in cell hydrolysates.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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