Abstract
Diazouracil (DZU) was shown to increase the mean cell volume of several bacterial species. The filaments which it induced with Escherichia coli B and Lactobacillus casei were examined in section by electron microscopy. In addition to the inhibition of division of E. coli by DZU, its effects were studied on mass increase, viability, and deoxyribonucleic acid, ribonucleic acid, and protein synthesis. The dependence of cell age on the division-inhibitory effect of DZU was examined in synchronous cultures. The division-inhibitory effect was not reversed by pantoyllactone, an antagonist of ultraviolet filamentation, or by l-tyrosine, which had been reported to antagonize DZU activity.
Full text
PDF









Images in this article
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- ADLER H. I., HARDIGREE A. A. ANALYSIS OF A GENE CONTROLLING CELL DIVISION AND SENSITIVITY TO RADIATION IN ESCHERICHIA COLI. J Bacteriol. 1964 Mar;87:720–726. doi: 10.1128/jb.87.3.720-726.1964. [DOI] [PMC free article] [PubMed] [Google Scholar]
- ADLER H. I., HARDIGREE A. A. POSTIRRADIATION GROWTH, DIVISION, AND RECOVERY IN BACTERIA. Radiat Res. 1965 May;25:92–102. [PubMed] [Google Scholar]
- DEERING R. A. Studies on division inhibition and filament formation of Escherichia coli by ultraviolet light. J Bacteriol. 1958 Aug;76(2):123–130. doi: 10.1128/jb.76.2.123-130.1958. [DOI] [PMC free article] [PubMed] [Google Scholar]
- GRULA M. M., GRULA E. A. Cell division in a species of Erwinia IV. Metabolic blocks in panothenate biosynthesis and their relationship to inhibition of cell division. J Bacteriol. 1962 May;83:989–997. doi: 10.1128/jb.83.5.989-997.1962. [DOI] [PMC free article] [PubMed] [Google Scholar]
- HELMSTETTER C. E., CUMMINGS D. J. BACTERIAL SYNCHRONIZATION BY SELECTION OF CELLS AT DIVISION. Proc Natl Acad Sci U S A. 1963 Oct;50:767–774. doi: 10.1073/pnas.50.4.767. [DOI] [PMC free article] [PubMed] [Google Scholar]
- HOWARD-FLANDERS P., SIMSON E., THERIOT L. A LOCUS THAT CONTROLS FILAMENT FORMATION AND SENSITIVITY TO RADIATION IN ESCHERICHIA COLI K-12. Genetics. 1964 Feb;49:237–246. doi: 10.1093/genetics/49.2.237. [DOI] [PMC free article] [PubMed] [Google Scholar]
- KUBITSCHEK H. E. Normal distribution of cell generation rate. Exp Cell Res. 1962 Mar;26:439–450. doi: 10.1016/0014-4827(62)90150-7. [DOI] [PubMed] [Google Scholar]
- Koch A. L. On evidence supporting a deterministic process of bacterial growth. J Gen Microbiol. 1966 Apr;43(1):1–5. doi: 10.1099/00221287-43-1-1. [DOI] [PubMed] [Google Scholar]
- Koch A. L. Theory of the angular dependence of light scattered by bacteria and similar-sized biological objects. J Theor Biol. 1968 Jan;18(1):133–156. doi: 10.1016/0022-5193(68)90174-4. [DOI] [PubMed] [Google Scholar]
- LOVELESS L. E., SPOERL E., WEISMAN T. H. A survey of effects of chemicals on division and growth of yeast and Escherichia coli. J Bacteriol. 1954 Dec;68(6):637–644. doi: 10.1128/jb.68.6.637-644.1954. [DOI] [PMC free article] [PubMed] [Google Scholar]
- LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
- LUFT J. H. Improvements in epoxy resin embedding methods. J Biophys Biochem Cytol. 1961 Feb;9:409–414. doi: 10.1083/jcb.9.2.409. [DOI] [PMC free article] [PubMed] [Google Scholar]
- MASTER R. W. POSSIBLE SYNTHESIS OF POLYRIBONUCLEOTIDES OF KNOWN BASE-TRIPLET SEQUENCES. Nature. 1965 Apr 3;206:93–93. doi: 10.1038/206093b0. [DOI] [PubMed] [Google Scholar]
- Ryter A. Association of the nucleus and the membrane of bacteria: a morphological study. Bacteriol Rev. 1968 Mar;32(1):39–54. doi: 10.1128/br.32.1.39-54.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
- SCHAECHTER M., WILLIAMSON J. P., HOOD J. R., Jr, KOCH A. L. Growth, cell and nuclear divisions in some bacteria. J Gen Microbiol. 1962 Nov;29:421–434. doi: 10.1099/00221287-29-3-421. [DOI] [PubMed] [Google Scholar]
- SWANN M. M. The control of cell division; a review. I. General mechanisms. Cancer Res. 1957 Sep;17(8):727–757. [PubMed] [Google Scholar]
- Shands J. W., Jr Embedding free-floating cells and microscopic particles; serum albumin coagulum-epoxy resin. Stain Technol. 1968 Jan;43(1):15–17. doi: 10.3109/10520296809115036. [DOI] [PubMed] [Google Scholar]
- Stárka J., Moravová J. Cellular division of penicillin-induced filaments of Escherichia coli. Folia Microbiol (Praha) 1967;12(3):240–247. doi: 10.1007/BF02868738. [DOI] [PubMed] [Google Scholar]
- WEISMAN T. H., LOVELESS L. E. Chemical inhibition of cell division of Escherichia coli by diazo compounds and antagonism by tyrosine. Proc Soc Exp Biol Med. 1954 Jun;86(2):268–273. doi: 10.3181/00379727-86-21071. [DOI] [PubMed] [Google Scholar]
- Walker J. R., Pardee A. B. Evidence for a relationship between deoxyribonucleic acid metabolism and septum formation in Escherichia coli. J Bacteriol. 1968 Jan;95(1):123–131. doi: 10.1128/jb.95.1.123-131.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]