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. 1990 Aug;64(8):3598–3604. doi: 10.1128/jvi.64.8.3598-3604.1990

Simian foamy virus type 1 is a retrovirus which encodes a transcriptional transactivator.

A Mergia 1, K E Shaw 1, E Pratt-Lowe 1, P A Barry 1, P A Luciw 1
PMCID: PMC249652  PMID: 2370676

Abstract

Simian foamy viruses, members of the spumavirus subfamily of retroviruses, are found in a variety of nonhuman primates and, as yet, remain to be characterized with respect to genetic structure and regulation of viral gene expression. The genome of simian foamy virus type 1 (SFV-1), an isolate from rhesus macaques, has been molecularly cloned, and the role of the viral long terminal repeat (LTR) in transcriptional control has been investigated. The SFV-1 LTR is 1,621 base pairs long, and sequence comparisons with human foamy virus revealed a pattern of clustered homology. A cap site in the LTR was identified by analysis of SFV-1 transcripts in infected cells. Transient expression assays in cell lines representing several species and different cell types showed that the SFV-1 LTR has low basal activity in uninfected cells, whereas LTR-directed expression is greatly increased in cells infected with SFV-1. This transactivation is mediated by a mechanism involving increases in steady-state levels of viral transcripts. Thus, the SFV-1 genome encodes a transactivator that functions on the LTR at the transcriptional level.

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Selected References

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  1. Benzair A. B., Rhodes-Feuillette A., Emanoïl-Ravicovitch R., Peries J. Reverse transcriptase from simian foamy virus serotype 1: purification and characterization. J Virol. 1982 Nov;44(2):720–724. doi: 10.1128/jvi.44.2.720-724.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Chomczynski P., Sacchi N. Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction. Anal Biochem. 1987 Apr;162(1):156–159. doi: 10.1006/abio.1987.9999. [DOI] [PubMed] [Google Scholar]
  3. Cullen B. R., Greene W. C. Regulatory pathways governing HIV-1 replication. Cell. 1989 Aug 11;58(3):423–426. doi: 10.1016/0092-8674(89)90420-0. [DOI] [PubMed] [Google Scholar]
  4. Cullen B. R. Trans-activation of human immunodeficiency virus occurs via a bimodal mechanism. Cell. 1986 Sep 26;46(7):973–982. doi: 10.1016/0092-8674(86)90696-3. [DOI] [PubMed] [Google Scholar]
  5. Flügel R. M., Rethwilm A., Maurer B., Darai G. Nucleotide sequence analysis of the env gene and its flanking regions of the human spumaretrovirus reveals two novel genes. EMBO J. 1987 Jul;6(7):2077–2084. doi: 10.1002/j.1460-2075.1987.tb02473.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Green P. L., Chen I. S. Regulation of human T cell leukemia virus expression. FASEB J. 1990 Feb 1;4(2):169–175. doi: 10.1096/fasebj.4.2.2404818. [DOI] [PubMed] [Google Scholar]
  7. Hooks J. J., Detrick-Hooks B. Simian foamy virus-induced immunosuppression in rabbits. J Gen Virol. 1979 Aug;44(2):383–390. doi: 10.1099/0022-1317-44-2-383. [DOI] [PubMed] [Google Scholar]
  8. Johnson P. F., McKnight S. L. Eukaryotic transcriptional regulatory proteins. Annu Rev Biochem. 1989;58:799–839. doi: 10.1146/annurev.bi.58.070189.004055. [DOI] [PubMed] [Google Scholar]
  9. Kupiec J. J., Tobaly-Tapiero J., Canivet M., Santillana-Hayat M., Flügel R. M., Périès J., Emanoil-Ravier R. Evidence for a gapped linear duplex DNA intermediate in the replicative cycle of human and simian spumaviruses. Nucleic Acids Res. 1988 Oct 25;16(20):9557–9565. doi: 10.1093/nar/16.20.9557. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Lopata M. A., Cleveland D. W., Sollner-Webb B. High level transient expression of a chloramphenicol acetyl transferase gene by DEAE-dextran mediated DNA transfection coupled with a dimethyl sulfoxide or glycerol shock treatment. Nucleic Acids Res. 1984 Jul 25;12(14):5707–5717. doi: 10.1093/nar/12.14.5707. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Maurer B., Bannert H., Darai G., Flügel R. M. Analysis of the primary structure of the long terminal repeat and the gag and pol genes of the human spumaretrovirus. J Virol. 1988 May;62(5):1590–1597. doi: 10.1128/jvi.62.5.1590-1597.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Mergia A., Shaw K. E., Lackner J. E., Luciw P. A. Relationship of the env genes and the endonuclease domain of the pol genes of simian foamy virus type 1 and human foamy virus. J Virol. 1990 Jan;64(1):406–410. doi: 10.1128/jvi.64.1.406-410.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Narayan O., Clements J. E. Biology and pathogenesis of lentiviruses. J Gen Virol. 1989 Jul;70(Pt 7):1617–1639. doi: 10.1099/0022-1317-70-7-1617. [DOI] [PubMed] [Google Scholar]
  14. Nerenberg M., Hinrichs S. H., Reynolds R. K., Khoury G., Jay G. The tat gene of human T-lymphotropic virus type 1 induces mesenchymal tumors in transgenic mice. Science. 1987 Sep 11;237(4820):1324–1329. doi: 10.1126/science.2888190. [DOI] [PubMed] [Google Scholar]
  15. Neumann-Haefelin D., Schweizer M., Corsten B., Matz B. Detection and characterization of infectious DNA intermediates of a primary foamy virus. J Gen Virol. 1986 Sep;67(Pt 9):1993–1999. doi: 10.1099/0022-1317-67-9-1993. [DOI] [PubMed] [Google Scholar]
  16. Nordeen S. K., Green P. P., 3rd, Fowlkes D. M. A rapid, sensitive, and inexpensive assay for chloramphenicol acetyltransferase. DNA. 1987 Apr;6(2):173–178. doi: 10.1089/dna.1987.6.173. [DOI] [PubMed] [Google Scholar]
  17. Pedersen N. C., Pool R. R., O'Brien T. Feline chronic progressive polyarthritis. Am J Vet Res. 1980 Apr;41(4):522–535. [PubMed] [Google Scholar]
  18. Peterlin B. M., Luciw P. A., Barr P. J., Walker M. D. Elevated levels of mRNA can account for the trans-activation of human immunodeficiency virus. Proc Natl Acad Sci U S A. 1986 Dec;83(24):9734–9738. doi: 10.1073/pnas.83.24.9734. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Selby M. J., Bain E. S., Luciw P. A., Peterlin B. M. Structure, sequence, and position of the stem-loop in tar determine transcriptional elongation by tat through the HIV-1 long terminal repeat. Genes Dev. 1989 Apr;3(4):547–558. doi: 10.1101/gad.3.4.547. [DOI] [PubMed] [Google Scholar]
  20. Sharp P. A., Marciniak R. A. HIV TAR: an RNA enhancer? Cell. 1989 Oct 20;59(2):229–230. doi: 10.1016/0092-8674(89)90279-1. [DOI] [PubMed] [Google Scholar]
  21. Stancek D., Stanceková-Gressnerová M., Janotka M., Hnilica P., Oravec D. Isolation and some serological and epidemiological data on the viruses recovered from patients with subacute thyroiditis de Quervain. Med Microbiol Immunol. 1975;161(2):133–144. doi: 10.1007/BF02121755. [DOI] [PubMed] [Google Scholar]
  22. Weiss R. A. Foamy retroviruses. A virus in search of a disease. Nature. 1988 Jun 9;333(6173):497–498. doi: 10.1038/333497a0. [DOI] [PubMed] [Google Scholar]

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