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. 1990 Aug;64(8):4021–4024. doi: 10.1128/jvi.64.8.4021-4024.1990

Identification of human rotavirus serotype by hybridization to polymerase chain reaction-generated probes derived from a hyperdivergent region of the gene encoding outer capsid protein VP7.

J Flores 1, J Sears 1, I P Schael 1, L White 1, D Garcia 1, C Lanata 1, A Z Kapikian 1
PMCID: PMC249704  PMID: 2164610

Abstract

We have synthesized 32P-labeled hybridization probes from a hyperdivergent region (nucleotides 51 to 392) of the rotavirus gene encoding the VP7 glycoprotein by using the polymerase chain reaction method. Both RNA (after an initial reverse transcription step) and cloned cDNA from human rotavirus serotypes 1 through 4 could be used as templates to amplify this region. High-stringency hybridization of each of the four probes to rotavirus RNAs dotted on nylon membranes allowed the specific detection of corresponding sequences and thus permitted identification of the serotype of the strains dotted. The procedure was useful when applied to rotaviruses isolated from field studies.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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