Skip to main content
PMC home page
Journal of Virology logoLink to Journal of Virology
. 1990 Aug;64(8):4029–4032. doi: 10.1128/jvi.64.8.4029-4032.1990

Envelope glycoprotein of avian hemangioma retrovirus induces a thrombogenic surface on human and bovine endothelial cells.

N Resnick-Roguel 1, A Eldor 1, H Burstein 1, E Hy-Am 1, I Vlodavsky 1, A Panet 1, M A Blajchman 1, M Kotler 1
PMCID: PMC249706  PMID: 2164612

Abstract

Vascular endothelial cells are a target for blood-borne pathogens which may affect their integrity and thromboresistant properties. Here, we report that cultured bovine and human endothelial cells lose their thromboresistance following interaction with the avian hemangioma-inducing retrovirus. We show that the envelope (env) gene product, glycoprotein 85, is responsible for this effect, which appears soon after infection without viral replication or cell transformation. Induction of thrombogenicity is associated with a reduction in prostacyclin release and increased expression of tissue factor. These observations may explain the occurrence of thrombosis frequently observed in association with the hemangiosarcomas induced by avian hemangioma-inducing retrovirus. These unique endothelial cell-virus interactions may also be a model for the pathogenesis of various vascular diseases.

Full text

PDF
4029

Images in this article

4030Image
on p.4030

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Adam E., Melnick J. L., Probtsfield J. L., Petrie B. L., Burek J., Bailey K. R., McCollum C. H., DeBakey M. E. High levels of cytomegalovirus antibody in patients requiring vascular surgery for atherosclerosis. Lancet. 1987 Aug 8;2(8554):291–293. doi: 10.1016/s0140-6736(87)90888-9. [DOI] [PubMed] [Google Scholar]
  2. Altaner C., Temin H. M. Carcinogenesis by RNA sarcoma viruses. XII. A quantitative study of infection of rat cells in vitro by avian sarcoma viruses. Virology. 1970 Jan;40(1):118–134. doi: 10.1016/0042-6822(70)90384-3. [DOI] [PubMed] [Google Scholar]
  3. Beilke M. A. Vascular endothelium in immunology and infectious disease. Rev Infect Dis. 1989 Mar-Apr;11(2):273–283. doi: 10.1093/clinids/11.2.273. [DOI] [PubMed] [Google Scholar]
  4. Benditt E. P., Barrett T., McDougall J. K. Viruses in the etiology of atherosclerosis. Proc Natl Acad Sci U S A. 1983 Oct;80(20):6386–6389. doi: 10.1073/pnas.80.20.6386. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Bloem L. J., Chen L., Konigsberg W. H., Bach R. Serum stimulation of quiescent human fibroblasts induces the synthesis of tissue factor mRNA followed by the appearance of tissue factor antigen and procoagulant activity. J Cell Physiol. 1989 May;139(2):418–423. doi: 10.1002/jcp.1041390226. [DOI] [PubMed] [Google Scholar]
  6. Cosgriff T. M. Viruses and hemostasis. Rev Infect Dis. 1989 May-Jun;11 (Suppl 4):S672–S688. doi: 10.1093/clinids/11.supplement_4.s672. [DOI] [PubMed] [Google Scholar]
  7. Einhorn S., Eldor A., Vlodavsky I., Fuks Z., Panet A. Production and characterization of interferon from endothelial cells. J Cell Physiol. 1985 Feb;122(2):200–204. doi: 10.1002/jcp.1041220206. [DOI] [PubMed] [Google Scholar]
  8. Eldor A., Fridman R., Vlodavsky I., Hy-Am E., Fuks Z., Panet A. Interferon enhances prostacyclin production by cultured vascular endothelial cells. J Clin Invest. 1984 Jan;73(1):251–257. doi: 10.1172/JCI111198. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Eldor A., Vlodavsky I., Fuks Z., Muller T. H., Eisert W. G. Different effects of aspirin, dipyridamole and UD-CG 115 on platelet activation in a model of vascular injury: studies with extracellular matrix covered with endothelial cells. Thromb Haemost. 1986 Dec 15;56(3):333–339. [PubMed] [Google Scholar]
  10. Friedman H. M. Infection of endothelial cells by common human viruses. Rev Infect Dis. 1989 May-Jun;11 (Suppl 4):S700–S704. doi: 10.1093/clinids/11.supplement_4.s700. [DOI] [PubMed] [Google Scholar]
  11. Friedman H. M., Macarak E. J., MacGregor R. R., Wolfe J., Kefalides N. A. Virus infection of endothelial cells. J Infect Dis. 1981 Feb;143(2):266–273. doi: 10.1093/infdis/143.2.266. [DOI] [PubMed] [Google Scholar]
  12. Kotler M. Interactions of avian sarcoma virus with rat embryo cells in cell culture. J Gen Virol. 1971 Sep;12(3):199–206. doi: 10.1099/0022-1317-12-3-199. [DOI] [PubMed] [Google Scholar]
  13. Mantovani A., Dejana E. Cytokines as communication signals between leukocytes and endothelial cells. Immunol Today. 1989 Nov;10(11):370–375. doi: 10.1016/0167-5699(89)90270-3. [DOI] [PubMed] [Google Scholar]
  14. Oldstone M. B. Viruses can cause disease in the absence of morphological evidence of cell injury: implication for uncovering new diseases in the future. J Infect Dis. 1989 Mar;159(3):384–389. doi: 10.1093/infdis/159.3.384. [DOI] [PubMed] [Google Scholar]
  15. Resnick-Roguel N., Burstein H., Hamburger J., Panet A., Eldor A., Vlodavsky I., Kotler M. Cytocidal effect caused by the envelope glycoprotein of a newly isolated avian hemangioma-inducing retrovirus. J Virol. 1989 Oct;63(10):4325–4330. doi: 10.1128/jvi.63.10.4325-4330.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Ross R. The pathogenesis of atherosclerosis--an update. N Engl J Med. 1986 Feb 20;314(8):488–500. doi: 10.1056/NEJM198602203140806. [DOI] [PubMed] [Google Scholar]
  17. Samuelsson B., Goldyne M., Granström E., Hamberg M., Hammarström S., Malmsten C. Prostaglandins and thromboxanes. Annu Rev Biochem. 1978;47:997–1029. doi: 10.1146/annurev.bi.47.070178.005025. [DOI] [PubMed] [Google Scholar]
  18. Vogel J., Hinrichs S. H., Reynolds R. K., Luciw P. A., Jay G. The HIV tat gene induces dermal lesions resembling Kaposi's sarcoma in transgenic mice. Nature. 1988 Oct 13;335(6191):606–611. doi: 10.1038/335606a0. [DOI] [PubMed] [Google Scholar]
  19. Williams R. L., Risau W., Zerwes H. G., Drexler H., Aguzzi A., Wagner E. F. Endothelioma cells expressing the polyoma middle T oncogene induce hemangiomas by host cell recruitment. Cell. 1989 Jun 16;57(6):1053–1063. doi: 10.1016/0092-8674(89)90343-7. [DOI] [PubMed] [Google Scholar]

Articles from Journal of Virology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES