Abstract
A factor in bacterial cell extracts which induces cell division in filaments of Escherichia coli produced by irradiation was found to be associated with a heat-labile particulate fraction which sediments at about 100S. Frozen or lyophilized samples of the cell extracts were stable for considerable periods of time. Fractions purified by centrifugation contained 2% of the total protein of the extract but no measurable ribonucleic acid or deoxyribonucleic acid. The extracts were inactivated by incubation with phospholipase A, lipases, and detergents, but not by incubation with selected nucleases and proteases.
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- ADLER H. I., COPELAND J. C. Genetic analysis of radiation response in Escherichia coli. Genetics. 1962 Jun;47:701–712. doi: 10.1093/genetics/47.6.701. [DOI] [PMC free article] [PubMed] [Google Scholar]
- ADLER H. I., HARDIGREE A. A. POSTIRRADIATION GROWTH, DIVISION, AND RECOVERY IN BACTERIA. Radiat Res. 1965 May;25:92–102. [PubMed] [Google Scholar]
- ADLER H. I., HASKINS S. D. Heterogeneity of cultures of Escherichia coli B/r. Nature. 1960 Oct 15;188:249–251. doi: 10.1038/188249a0. [DOI] [PubMed] [Google Scholar]
- ALPER T., GILLIES N. E. The relationship between growth and survival after irradiation of Escherichia coli strain B and two resistant mutants. J Gen Microbiol. 1960 Feb;22:113–128. doi: 10.1099/00221287-22-1-113. [DOI] [PubMed] [Google Scholar]
- ANDERSON N. G. Analytical techniques for cell fractions. II. A spectrophotometric column monitoring system. Anal Biochem. 1962 Oct;4:269–283. doi: 10.1016/0003-2697(62)90088-x. [DOI] [PubMed] [Google Scholar]
- Adler H. I., Fisher W. D., Hardigree A. A., Stapleton G. E. Repair of radiation-induced damage to the cell division mechanism of Escherichia coli. J Bacteriol. 1966 Feb;91(2):737–742. doi: 10.1128/jb.91.2.737-742.1966. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Anderson N. G., Harris W. W., Barber A. A., Rankin C. T., Jr, Candler E. L. Separation of subcellular components and viruses by combined rate- and isopycnic-zonal centrifugation. Natl Cancer Inst Monogr. 1966 Jun;21:253–283. [PubMed] [Google Scholar]
- Anderson N. G., Waters D. A., Fisher W. D., Cline G. B., Nunley C. E., Elrod L. H., Rankin C. T., Jr Analytical techniques for cell fractions. V. Characteristics of the B-XIV and B-XV zonal centrifuge rotors. Anal Biochem. 1967 Nov;21(2):235–252. doi: 10.1016/0003-2697(67)90185-6. [DOI] [PubMed] [Google Scholar]
- BURTON K. A study of the conditions and mechanism of the diphenylamine reaction for the colorimetric estimation of deoxyribonucleic acid. Biochem J. 1956 Feb;62(2):315–323. doi: 10.1042/bj0620315. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Cho N., Barringer H. P., Amburgey J. W., Cline G. B., Anderson N. G., McCauley L. L., Stevens R. H., Swartout W. M. Problems in biocontainment. Natl Cancer Inst Monogr. 1966 Jun;21:485–502. [PubMed] [Google Scholar]
- DELAPORTE B. La restauration par voisinage chez des bactéries irradiées par des rayons X. Ann Inst Pasteur (Paris) 1956 Nov;91(5):727–735. [PubMed] [Google Scholar]
- FISHER W. D., CLINE G. B., ANDERSON N. G. DENSITY GRADIENT CENTRIFUGATION IN ANGLE-HEAD ROTORS. Anal Biochem. 1964 Dec;9:477–482. doi: 10.1016/0003-2697(64)90209-x. [DOI] [PubMed] [Google Scholar]
- GRULA E. A., GRULA M. M. Cell division in a species of Erwinia III. Reversal of inhibition of cell division caused by D-amino acids, penicillin, and ultraviolet light. J Bacteriol. 1962 May;83:981–988. doi: 10.1128/jb.83.5.981-988.1962. [DOI] [PMC free article] [PubMed] [Google Scholar]
- IMAI Y., SATO R. Solubilization of aromatic hydroxylase system of liver microsomes and requirement of lipid-like factor. Biochim Biophys Acta. 1960 Jul 29;42:164–165. doi: 10.1016/0006-3002(60)90767-8. [DOI] [PubMed] [Google Scholar]
- JAGGER J., STAFFORD R. S. EVIDENCE FOR TWO MECHANISMS OF PHOTOREACTIVATION IN ESCHERICHIA COLI B. Biophys J. 1965 Jan;5:75–88. doi: 10.1016/s0006-3495(65)86703-0. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Korgaonkar K. S., Raut S. L. Reactivation in UV inactivated E. coli K12 by cell-free extracts. Curr Mod Biol. 1967 Nov;1(4):291–298. doi: 10.1016/0303-2647(67)90009-3. [DOI] [PubMed] [Google Scholar]
- LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
- VOLKIN E., COHN W. E. Estimation of nucleic acids. Methods Biochem Anal. 1954;1:287–305. doi: 10.1002/9780470110171.ch11. [DOI] [PubMed] [Google Scholar]