Abstract
In examining the medium used in cultivation of Mycoplasma for deoxyribonucleic acid isolation, it was found that an aggregate was present which sedimented with the organisms and which was ethyl alcohol-precipitated during deoxyribonucleic acid purification. To eliminate the contaminating material, a method was devised to obtain only the dialyzable constituents of the medium. This report describes the preparation of a dialysate of soy peptone-yeast extract. The medium, obtained by immersion of the encased dehydrated ingredients in sodium chloride solution for 5.5 hr at approximately 80 C, has been employed as the basal medium for cultivation of a number of Mycoplasma species. Comparative growth curves of two saprophytic strains and two parasitic species indicated that multiplication in dialysate, with suitable supplement, followed the pattern typical of the common eubacteria. Thus, by elimination of the sediment which occurred in nondialyzed medium, Mycoplasma could be concentrated without concomitant accumulation of contaminating macromolecules.
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Selected References
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- Anderson D. L., Pollock M. E., Brower L. F. Morphology of Mycoplasma laidlawii type A. I. Comparison of electron microscopic counts with colony-forming units. J Bacteriol. 1965 Dec;90(6):1764–1767. doi: 10.1128/jb.90.6.1764-1767.1965. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Deeb B. J., Kenny G. E. Characterization of Mycoplasma pulmonis variants isolated from rabbits. I. Identification and properties of isolates. J Bacteriol. 1967 Apr;93(4):1416–1424. doi: 10.1128/jb.93.4.1416-1424.1967. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Herold J. D., Schultz J. S., Gerhardt P. Differential dialysis culture for separation and concentration of a macromolecular product. Appl Microbiol. 1967 Sep;15(5):1192–1197. doi: 10.1128/am.15.5.1192-1197.1967. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Ogata M., Ota T., Obara A., Pan I. Z. [Investigation on growth media for Mycoplasma: evaluation of infusions, peptones, sera, yeast extracts and other supplements]. Nihon Juigaku Zasshi. 1967 Oct;29(5):259–271. doi: 10.1292/jvms1939.29.259. [DOI] [PubMed] [Google Scholar]
- POLLOCK M. E., TREADWELL P. E., KENNY G. E. MAMMALIAN CELL CULTURES CONTAMINATED WITH PLEUROPNEUMONIA-LIKE ORGANISMS. II. EFFECT OF PPLO ON CELL MORPHOLOGY IN ESTABLISHED MONOLAYER CULTURES. Exp Cell Res. 1963 Aug;31:321–328. doi: 10.1016/0014-4827(63)90009-0. [DOI] [PubMed] [Google Scholar]
- Pollock M. E. Use of dialyzing culture technique for high yield of Mycoplasma. J Bacteriol. 1965 Dec;90(6):1682–1685. doi: 10.1128/jb.90.6.1682-1685.1965. [DOI] [PMC free article] [PubMed] [Google Scholar]