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. 1969 Feb;97(2):557–560. doi: 10.1128/jb.97.2.557-560.1969

Deoxyribonucleic Acid Base Composition in the Taxonomy of Staphylococcus

Francis L Garrity 1, Barbara Detrick 1, E R Kennedy 1
PMCID: PMC249727  PMID: 4886284

Abstract

Twenty-four strains of Staphylococcus aureus, including eight known mutants of S. aureus and strains growing under a variety of environmental conditions or exposed to a number of physical and chemical agents, maintained a remarkably narrow range of guanine plus cytosine (GC) content (32.4 to 35.1%). The wide range of GC content (30.7 to 40%) reported in the literature was due to the variety of methods and calculations used rather than to any substantial variation in base composition. The UV-2 “mutant” (ATCC 13680) with a GC content of 67.6% reported to be derived from S. aureus (ATCC 13679) was a species of Corynebacterium. The data presented were consistent with the concept that base composition changes only to a very slight degree by mutation.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Auletta A. E., Kennedy E. R. Deoxyribonucleic acid base composition of some members of the Micrococcaceae. J Bacteriol. 1966 Jul;92(1):28–34. doi: 10.1002/path.1700920103. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. BEINING P. R., KENNEDY E. R. CHARACTERISTICS OF A STRAIN OF STAPHYLOCOCCUS AUREUS GROWN IN VIVO AND IN VITRO. J Bacteriol. 1963 Apr;85:732–741. doi: 10.1128/jb.85.4.732-741.1963. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Baird-Parker A. C. Staphylococci and their classification. Ann N Y Acad Sci. 1965 Jul 23;128(1):4–25. doi: 10.1111/j.1749-6632.1965.tb11626.x. [DOI] [PubMed] [Google Scholar]
  4. FREDERICQ E., OTH A., FONTAINE F. The ultraviolet spectrum of deoxyribonucleic acids and their constituents. J Mol Biol. 1961 Feb;3:11–17. doi: 10.1016/s0022-2836(61)80003-x. [DOI] [PubMed] [Google Scholar]
  5. Felsenfeld G., Hirschman S. Z. A neighbor-interaction analysis of the hypochromism and spectra of DNA. J Mol Biol. 1965 Sep;13(2):407–427. doi: 10.1016/s0022-2836(65)80106-1. [DOI] [PubMed] [Google Scholar]
  6. GAUSE G. G., LOSHKAREVA N. P., ZBARSKY I. B., GAUSE G. F. DEOXYRIBONUCLEIC ACID BASE COMPOSITION IN CERTAIN BACTERIA AND THEIR MUTANTS WITH IMPAIRED RESPIRATION. Nature. 1964 Aug 8;203:598–599. doi: 10.1038/203598a0. [DOI] [PubMed] [Google Scholar]
  7. HUGH R., LEIFSON E. The taxonomic significance of fermentative versus oxidative metabolism of carbohydrates by various gram negative bacteria. J Bacteriol. 1953 Jul;66(1):24–26. doi: 10.1128/jb.66.1.24-26.1953. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Hirschman S. Z., Felsenfeld G. Determination of DNA composition and concentration by spectral analysis. J Mol Biol. 1966 Apr;16(2):347–358. doi: 10.1016/s0022-2836(66)80178-x. [DOI] [PubMed] [Google Scholar]
  9. Klesius P. H., Schuhardt V. T. Use of lysostaphin in the isolation of highly polymerized deoxyribonucleic acid and in the taxonomy of aerobic Micrococcaceae. J Bacteriol. 1968 Mar;95(3):739–743. doi: 10.1128/jb.95.3.739-743.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. MARMUR J., DOTY P. Determination of the base composition of deoxyribonucleic acid from its thermal denaturation temperature. J Mol Biol. 1962 Jul;5:109–118. doi: 10.1016/s0022-2836(62)80066-7. [DOI] [PubMed] [Google Scholar]
  11. SCHILDKRAUT C. L., MARMUR J., DOTY P. Determination of the base composition of deoxyribonucleic acid from its buoyant density in CsCl. J Mol Biol. 1962 Jun;4:430–443. doi: 10.1016/s0022-2836(62)80100-4. [DOI] [PubMed] [Google Scholar]
  12. Silvestri L. G., Hill L. R. Agreement Between Deoxyribonucleic Acid Base Composition and Taxometric Classification of Gram-Positive Cocci. J Bacteriol. 1965 Jul;90(1):136–140. doi: 10.1128/jb.90.1.136-140.1965. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Stuart C. A., Van Stratum E., Rustigian R. Further Studies on Urease Production by Proteus and Related Organisms. J Bacteriol. 1945 May;49(5):437–444. doi: 10.1128/jb.49.5.437-444.1945. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. WEED L. L. EFFECTS OF COPPER ON BACILLUS SUBTILIS. J Bacteriol. 1963 May;85:1003–1010. doi: 10.1128/jb.85.5.1003-1010.1963. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. WYATT G. R. The purine and pyrimidine composition of deoxypentose nucleic acids. Biochem J. 1951 May;48(5):584–590. doi: 10.1042/bj0480584. [DOI] [PMC free article] [PubMed] [Google Scholar]

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