Abstract
The synthetic activities of isolated cells of the meningopneumonitis strain (MN) of Chlamydia psittaci were investigated and further observations were made on their catabolic reactions. These observations included the demonstration of CO2 production from aspartate in the presence of pyruvate and the formation of pyruvate from glucose-6-phosphate. Both reactions were enhanced by added adenosine triphosphate (ATP). Of a large number of compounds tested, only glucose-6-phosphate, pyruvate, aspartate, and isoleucine were shown to furnish carbons that were incorporated into molecules precipitated by trichloroacetic acid. The reactions with pyruvate, aspartate, and isoleucine were dependent entirely, or almost entirely, on added ATP, and the reaction with glucose-6-phosphate was enhanced by ATP. Except for CO2, which greatly stimulated the reactions, the addition of a number of other compounds or a combination of compounds, such as cofactors, amino acids, and purine and pyrimidine bases, did not greatly affect incorporation. About 95% of the activity of the trichloroacetic acid precipitates was recovered in the chloroform-methanol soluble fraction.
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