Abstract
The effects of cycloheximide and 5-fluorouracil on the formation of low-molecular-weight ribonucleic acid (RNA) in yeast were investigated. Both compounds were found to affect the synthesis of low-molecular-weight RNA of ribosomal origin more than transfer RNA but less than the high-molecular-weight ribosomal RNA. 5-Fluorouracil-containing transfer RNA was separated from normal transfer RNA by chromatography on diethylaminoethyl cellulose at 80 C in the presence of 7 m urea.
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- Brown D. D., Weber C. S. Gene linkage by RNA-DNA hybridization. I. Unique DNA sequences homologous to 4 s RNA, 5 s RNA and ribosomal RNA. J Mol Biol. 1968 Jun 28;34(3):661–680. doi: 10.1016/0022-2836(68)90188-5. [DOI] [PubMed] [Google Scholar]
- Comb D. G., Zehavi-Willner T. Isolation, purification and properties of 5 s ribosomal RNA: a new species of cellular RNA. J Mol Biol. 1967 Feb 14;23(3):441–458. doi: 10.1016/s0022-2836(67)80117-7. [DOI] [PubMed] [Google Scholar]
- Galibert F., Larsen C. J., Lelong J. C., Boiron M. RNA of low molecular weight in ribosomes of mammalian cells. Nature. 1965 Sep 4;207(5001):1039–1041. doi: 10.1038/2071039a0. [DOI] [PubMed] [Google Scholar]
- HOLLEY R. W., APGAR J., DOCTOR B. P., FARROW J., MARINI M. A., MERRILL S. H. A simplified procedure for the preparation of tyrosine and valine-acceptor fractions of yeast "soluble ribonucleic acid". J Biol Chem. 1961 Jan;236:200–202. [PubMed] [Google Scholar]
- Knight E., Jr, Darnell J. E. Distribution of 5 s RNA in HeLa cells. J Mol Biol. 1967 Sep 28;28(3):491–502. doi: 10.1016/s0022-2836(67)80099-8. [DOI] [PubMed] [Google Scholar]
- Lowrie R. J., Bergquist P. L. Transfer ribonucleic acids from Escherichia coli treated with 5-fluorouracil. Biochemistry. 1968 May;7(5):1761–1770. doi: 10.1021/bi00845a021. [DOI] [PubMed] [Google Scholar]
- MARCOT-QUEIROZ J., JULIEN J., ROSSET R., MONIER R. LES ACIDES RIBONUCL'EIQUES DES RIBOSOMES DE LA LEVURE. Bull Soc Chim Biol (Paris) 1965;47:183–194. [PubMed] [Google Scholar]
- Mayo V. S., Andrean B. A., De Kloet S. R. Effects of cycloheximide and 5-fluorouracil on the synthesis of ribonucleic acid in yeast. Biochim Biophys Acta. 1968 Dec 17;169(2):297–305. doi: 10.1016/0005-2787(68)90038-5. [DOI] [PubMed] [Google Scholar]
- Perry R. P. The nucleolus and the synthesis of ribosomes. Prog Nucleic Acid Res Mol Biol. 1967;6:219–257. doi: 10.1016/s0079-6603(08)60528-0. [DOI] [PubMed] [Google Scholar]
- ROSSET R., MONIER R., JULIEN J. LES RIBOSOMES D'ESCHERICHIA COLI. I. MISE EN 'EVIDENCE D'UN RNA RIBOSOMIQUE DE FAIBLE POIDS MOL'ECULAIRE. Bull Soc Chim Biol (Paris) 1964;46:87–109. [PubMed] [Google Scholar]
- TOMLINSON R. V., TENER G. M. THE EFFECT OF UREA, FORMAMIDE, AND GLYCOLS ON THE SECONDARY BINDING FORCES IN THE ION-EXCHANGE CHROMATOGRAPHY OF POLYNUCLEOTIDES OF DEAE-CELLULOSE. Biochemistry. 1963 Jul-Aug;2:697–702. doi: 10.1021/bi00904a013. [DOI] [PubMed] [Google Scholar]
- Virmaux N., Mandel P., Urban P. F. Evidence of two soluble RNA types in eye lens. Biochem Biophys Res Commun. 1964 Jul 1;16(4):308–313. doi: 10.1016/0006-291x(64)90031-2. [DOI] [PubMed] [Google Scholar]
- de KLOET S., van WERMESKERKEN R., KONINGSBERGER V. V. Studies on protein synthesis by protoplasts of Saccharomyces carlsbergensis. I. The effect of ribonuclease on protein synthesis. Biochim Biophys Acta. 1961 Feb 12;47:138–143. doi: 10.1016/0006-3002(61)90838-1. [DOI] [PubMed] [Google Scholar]
- de Kloet S. R. Effects of 5-fluorouracil and 6-azauracil on the synthesis of ribonucleic acid and protein in Saccharomyces carlsbergensis. Biochem J. 1968 Jan;106(1):167–178. doi: 10.1042/bj1060167. [DOI] [PMC free article] [PubMed] [Google Scholar]
- de Kloet S. R. Ribonucleic acid synthesis in yeast. The effect of cycloheximide on the synthesis of ribonucleic acid in Saccharomyces carlsbergensis. Biochem J. 1966 Jun;99(3):566–581. doi: 10.1042/bj0990566. [DOI] [PMC free article] [PubMed] [Google Scholar]