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. 1969 Feb;97(2):848–860. doi: 10.1128/jb.97.2.848-860.1969

Chromosome Replication in Salmonella typhimurium1

Hilary Chan a, Karl G Lark a
PMCID: PMC249769  PMID: 4886297

Abstract

The replication of the Salmonella typhimurium chromosome was studied. As with E. coli 15T−, replication was sequential. After amino acid starvation, replication proceeded from a unique and heritable region of the chromosome. 5-Bromouracil, when substituted for thymine, did not disturb the sequence of replication nor did it initiate extra replication cycles. By labeling the origin and the terminus of the chromosome with 3H- and 14C-thymine, respectively, it was possible to determine that the rate of chain elongation decreases as the growth rate decreases. No gap in the replication cycle could be observed.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Abe M., Tomizawa J. Replication of the escherichia coli K12 chromosome. Proc Natl Acad Sci U S A. 1967 Nov;58(5):1911–1918. doi: 10.1073/pnas.58.5.1911. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Anraku N., Landman O. E. Control of the synthesis of macromolecules during amino acid and thymine starvation in Bacillus subtilis. J Bacteriol. 1968 May;95(5):1813–1827. doi: 10.1128/jb.95.5.1813-1827.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. BURTON K. A study of the conditions and mechanism of the diphenylamine reaction for the colorimetric estimation of deoxyribonucleic acid. Biochem J. 1956 Feb;62(2):315–323. doi: 10.1042/bj0620315. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Cerdá-Olmedo E., Hanawalt P. C., Guerola N. Mutagenesis of the replication point by nitrosoguanidine: map and pattern of replication of the Escherichia coli chromosome. J Mol Biol. 1968 May 14;33(3):705–719. doi: 10.1016/0022-2836(68)90315-x. [DOI] [PubMed] [Google Scholar]
  5. Cooper S., Helmstetter C. E. Chromosome replication and the division cycle of Escherichia coli B/r. J Mol Biol. 1968 Feb 14;31(3):519–540. doi: 10.1016/0022-2836(68)90425-7. [DOI] [PubMed] [Google Scholar]
  6. KELLENBERGER E., LARK K. G., BOLLE A. Amino acid dependent control of DNA synthesis in bacteria and vegetative phage. Proc Natl Acad Sci U S A. 1962 Oct 15;48:1860–1868. doi: 10.1073/pnas.48.10.1860. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. LARK K. G., REPKO T., HOFFMAN E. J. THE EFFECT OF AMINO ACID DEPRIVATION ON SUBSEQUENT DEOXYRIBONUCLEIC ACID REPLICATION. Biochim Biophys Acta. 1963 Sep 17;76:9–24. [PubMed] [Google Scholar]
  8. Lark C. Regulation of deoxyribonucleic acid synthesis in Escherichia coli: dependence on growth rates. Biochim Biophys Acta. 1966 Jun 22;119(3):517–525. doi: 10.1016/0005-2787(66)90128-6. [DOI] [PubMed] [Google Scholar]
  9. Lark K. G., Bird R. E. Segregation of the conserved units of DNA in Escherichia coli. Proc Natl Acad Sci U S A. 1965 Nov;54(5):1444–1450. doi: 10.1073/pnas.54.5.1444. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Lark K. G., Lark C. Regulation of chromosome replication in Escherichia coli: alternate replication of two chromosomes at slow growth rates. J Mol Biol. 1965 Aug;13(1):105–126. doi: 10.1016/s0022-2836(65)80083-3. [DOI] [PubMed] [Google Scholar]
  11. Lark K. G. Regulation of chromosome replication and segregation in bacteria. Bacteriol Rev. 1966 Mar;30(1):3–32. doi: 10.1128/br.30.1.3-32.1966. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Meselson M., Stahl F. W. THE REPLICATION OF DNA IN ESCHERICHIA COLI. Proc Natl Acad Sci U S A. 1958 Jul 15;44(7):671–682. doi: 10.1073/pnas.44.7.671. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. PRITCHARD R. H., LARK K. G. INDUCTION OF REPLICATION BY THYMINE STARVATION AT THE CHROMOSOME ORIGIN IN ESCHERICHIA COLI. J Mol Biol. 1964 Aug;9:288–307. doi: 10.1016/s0022-2836(64)80208-4. [DOI] [PubMed] [Google Scholar]
  14. SCHAECHTER M., MAALOE O., KJELDGAARD N. O. Dependency on medium and temperature of cell size and chemical composition during balanced grown of Salmonella typhimurium. J Gen Microbiol. 1958 Dec;19(3):592–606. doi: 10.1099/00221287-19-3-592. [DOI] [PubMed] [Google Scholar]
  15. Sanderson K. E. Revised linkage map of Salmonella typhimurium. Bacteriol Rev. 1967 Dec;31(4):354–372. doi: 10.1128/br.31.4.354-372.1967. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Wolf B., Newman A., Glaser D. A. On the origin and direction of replication of the Escherichia coli K12 chromosome. J Mol Biol. 1968 Mar 28;32(3):611–629. doi: 10.1016/0022-2836(68)90346-x. [DOI] [PubMed] [Google Scholar]
  17. ZINDER N. D. Sexuality and mating in salmonella. Science. 1960 Mar 25;131(3404):924–926. doi: 10.1126/science.131.3404.924. [DOI] [PubMed] [Google Scholar]

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