Abstract
Staphylococcal skin isolates belonging to phage type 71 were found to produce a bactericidal substance against some streptococci, pneumococci, and corynebacteria. Fifteen strains of group A streptococci belonging to 13 different M types, group C streptococci, and group D streptococci were uniformly inhibited on solid media and in broth by membrane-filtered supernatant fluids of the staphylococcal broth cultures. Inhibition of group G streptococci and other staphyloccoci was variable, and no inhibition of group B streptococci or of a variety of gram-negative rods was demonstrable. A quantitative variation observed to exist among susceptible organisms was a function of the inoculum size of the inhibited strains. The bactericidal substance could be detected best from 24 to 48 hr after inoculation of the staphylococci in tryptic soy broth or in a dialysate of tryptic soy broth. Little or no bactericidal activity was noted when the organisms were grown in several other liquid media. The bactericidal substance was nondialyzable and could be precipitated with ammonium sulfate. It was heat-stable and its activity was not altered within a pH range of 4.0 to 8.5. Pronase and three times crystallized trypsin totally abolished its activity. The concentrated ammonium sulfate precipitate could be fractionated on a Sephadex G-100 column into several peaks, with the bactericidal activity localized to a single peak.
Full text
PDF
Images in this article
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Anthony B. F., Perlman L. V., Wannamaker L. W. Skin infections and acute nephritis in American Indian children. Pediatrics. 1967 Feb;39(2):263–279. [PubMed] [Google Scholar]
- Anthony B. F., Wannamaker L. W. Bacterial interference in experimental burns. J Exp Med. 1967 Feb 1;125(2):319–336. doi: 10.1084/jem.125.2.319. [DOI] [PMC free article] [PubMed] [Google Scholar]
- BARROW G. I. Microbial antagonism by Staphylococcus aureus. J Gen Microbiol. 1963 Jun;31:471–481. doi: 10.1099/00221287-31-3-471. [DOI] [PubMed] [Google Scholar]
- Dajani A. S., Farah F. S., Kurban A. K. Bacterial etiology of superficial pyoderma in Lebanon. J Pediatr. 1968 Sep;73(3):431–435. doi: 10.1016/s0022-3476(68)80126-x. [DOI] [PubMed] [Google Scholar]
- PARKER M. T., SIMMONS L. E. The inhibition of Corynebacterium diphtheriae and other gram-positive organisms by Staphylococcus aureus. J Gen Microbiol. 1959 Oct;21:457–476. doi: 10.1099/00221287-21-2-457. [DOI] [PubMed] [Google Scholar]
- Ribble J. C. A mechanism of bacterial interference in vitro. J Immunol. 1967 Apr;98(4):716–723. [PubMed] [Google Scholar]
- SHINEFIELD H. R., RIBBLE J. C., EICHENWALD H. F., BORIS M., SUTHERLAND J. M. Bacterial interference: its effect on nursery-acquired infection with Staphylococcus aureus. V. An analysis and interpretation. Am J Dis Child. 1963 Jun;105:683–688. [PubMed] [Google Scholar]