Skip to main content
Journal of Bacteriology logoLink to Journal of Bacteriology
. 1969 Mar;97(3):1005–1008. doi: 10.1128/jb.97.3.1005-1008.1969

Isolation and Partial Characterization of a Staphylococcal Leukocyte Cytotaxin

W S Walker a,1, R L Barlet a, H M Kurtz a
PMCID: PMC249805  PMID: 5776515

Abstract

A factor which attracts rabbit polymorphonuclear leukocytes both in vitro and in vivo was isolated from the culture filtrate of a strain of Staphylococcus aureus. The activity of the chemotactic factor was independent of fresh serum and it was nondialyzable. Incubation of the factor with heat-inactivated human serum markedly inhibited its chemotactic property. The factor was heat-labile (80 C, 10 min) in the crude culture filtrate but was heat-stable when partially purified.

Full text

PDF
1005

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. BOYDEN S. The chemotactic effect of mixtures of antibody and antigen on polymorphonuclear leucocytes. J Exp Med. 1962 Mar 1;115:453–466. doi: 10.1084/jem.115.3.453. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. HARRIS H., LOTZ M. Factors influencing chemotaxis of the polymorphonuclear leucocyte. Br J Exp Pathol. 1956 Oct;37(5):477–480. [PMC free article] [PubMed] [Google Scholar]
  3. HARRIS H. Role of chemotaxis in inflammation. Physiol Rev. 1954 Jul;34(3):529–562. doi: 10.1152/physrev.1954.34.3.529. [DOI] [PubMed] [Google Scholar]
  4. HIRSCH J. G. Phagocytin: a bactericidal substance from polymorphonuclear leucocytes. J Exp Med. 1956 May 1;103(5):589–611. doi: 10.1084/jem.103.5.589. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Keller H. U., Sorkin E. Chemotaxis of leucocytes. Experientia. 1968 Jul 15;24(7):641–652. doi: 10.1007/BF02138287. [DOI] [PubMed] [Google Scholar]
  6. LEWIS J. H., FERGUSON J. H., JACKSON B. G. Bacterial activators (lysokinases) of the fibrinolytic enzyme system of serum. Proc Soc Exp Biol Med. 1949 Dec;72(3):703–706. doi: 10.3181/00379727-72-17549. [DOI] [PubMed] [Google Scholar]
  7. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  8. Taylor F. B., Jr, Ward P. A. Generation of chemotactic activity in rabbit serum by plasminogen-streptokinase mixtures. J Exp Med. 1967 Jul 1;126(1):149–158. doi: 10.1084/jem.126.1.149. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Uriel J. Méthode d'électrophorèse dans des gels d'acrylamide-agarose. Bull Soc Chim Biol (Paris) 1966;48(8):969–982. [PubMed] [Google Scholar]
  10. Ward P. A. A plasmin-split fragment of C'3 as a new chemotactic factor. J Exp Med. 1967 Aug 1;126(2):189–206. doi: 10.1084/jem.126.2.189. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Ward P. A., Cochrane C. G., Muller-Eberhard H. J. Further studies on the chemotactic factor of complement and its formation in vivo. Immunology. 1966 Aug;11(2):141–153. [PMC free article] [PubMed] [Google Scholar]
  12. Ward P. A., Lepow I. H., Newman L. J. Bacterial factors chemotactic for polymorphonuclear leukocytes. Am J Pathol. 1968 Apr;52(4):725–736. [PMC free article] [PubMed] [Google Scholar]

Articles from Journal of Bacteriology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES