Skip to main content
Journal of Bacteriology logoLink to Journal of Bacteriology
. 1969 Mar;97(3):1142–1148. doi: 10.1128/jb.97.3.1142-1148.1969

Target Analysis of Mitochondrial Genetic Units in Yeast1

N E Allen a, Anthony M MacQuillan a
PMCID: PMC249826  PMID: 5776520

Abstract

Target analyses of the induction of ρ mutants indicated that the cytoplasm of an aerobic nonrepressed yeast contains approximately 20 mutable units, whereas repressed cells exhibit approximately 3. Aerobic adaptation of fully repressed cells brings about an increase in these cytoplasmic units which continues after maximal respiration has been reached. This increase can be correlated with the increase in numbers of stained mitochondria. The data are interpreted with reference to mitochondrial deoxyribonucleic acid.

Full text

PDF

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. AVERS C. J., LIN F. H., PFEFFER C. R. HISTOCHEMICAL STUDIES OF MITOCHONDRIAL VARIATION DURING AEROBIC GROWTH OF RESPIRATION-NORMAL BAKER'S YEAST. J Histochem Cytochem. 1965 May-Jun;13:344–349. doi: 10.1177/13.5.344. [DOI] [PubMed] [Google Scholar]
  2. Atwood K. C., Norman A. On the Interpretation of Multi-Hit Survival Curves. Proc Natl Acad Sci U S A. 1949 Dec;35(12):696–709. doi: 10.1073/pnas.35.12.696. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Avers C. J. Distribution of cytochrome c peroxidase activity in wild-type and petite cells of bakers' yeast grown aerobically and anaerobically. J Bacteriol. 1967 Oct;94(4):1225–1235. doi: 10.1128/jb.94.4.1225-1235.1967. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Bartley W., Tustanoff E. R. The effect of metabolic inhibitors on the development of respiration in anaerobically grown yeast. Biochem J. 1966 Jun;99(3):599–603. doi: 10.1042/bj0990599. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Maroudas N. G., Wilkie D. Ultraviolet irradiation studies on the cytoplasmic determinant of the yeast mitochondrion. Biochim Biophys Acta. 1968 Oct 29;166(3):681–688. doi: 10.1016/0005-2787(68)90375-4. [DOI] [PubMed] [Google Scholar]
  6. Mounolou J. C., Jakob H., Slonimski P. P. Mitochondrial DNA from yeast "petite" mutants: specific changes in buoyant density corresponding to different cytoplasmic mutations. Biochem Biophys Res Commun. 1966 Jul 20;24(2):218–224. doi: 10.1016/0006-291x(66)90723-6. [DOI] [PubMed] [Google Scholar]
  7. NAGAI S., YANAGISHIMA N., NAGAI H. Advances in the study of respiration-deficient (RD) mutation in yeast and other microorganisms. Bacteriol Rev. 1961 Dec;25:404–426. doi: 10.1128/br.25.4.404-426.1961. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. OGUR M., ST. JOHN R., NAGAI S. Tetrazolium overlay technique for population studies of respiration deficiency in yeast. Science. 1957 May 10;125(3254):928–929. doi: 10.1126/science.125.3254.928. [DOI] [PubMed] [Google Scholar]
  9. Polakis E. S., Bartley W., Meek G. A. Changes in the structure and enzyme activity of Saccharomyces cerevisiae in response to changes in the environment. Biochem J. 1964 Feb;90(2):369–374. doi: 10.1042/bj0900369. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. RAUT C., SIMPSON W. L. The effect of x-rays and of ultraviolet light of different wavelengths on the production of cytochrome-deficient yeasts. Arch Biochem Biophys. 1955 Jul;57(1):218–228. doi: 10.1016/0003-9861(55)90194-6. [DOI] [PubMed] [Google Scholar]
  11. SCHATZ G. SUBCELLULAR PARTICLES CARRYING MITOCHONDRIAL ENZYMES IN ANAEROBICALLY-GROWN CELLS OF SACCHAROMYCES CEREVISIAE. Biochim Biophys Acta. 1965 Feb 22;96:342–345. [PubMed] [Google Scholar]
  12. SHERMAN F., SLONIMSKI P. P. RESPIRATION-DEFICIENT MUTANTS OF YEAST. II. BIOCHEMISTRY. Biochim Biophys Acta. 1964 Jul 15;90:1–15. doi: 10.1016/0304-4165(64)90113-8. [DOI] [PubMed] [Google Scholar]
  13. SHERMAN F. The effects of elevated temperatures on yeast. II. Induction of respiratory-deficient mutants. J Cell Comp Physiol. 1959 Aug;54:37–52. doi: 10.1002/jcp.1030540106. [DOI] [PubMed] [Google Scholar]
  14. SHORTMAN K., FUKUHARA H. METABOLISM OF RIBONUCLEIC ACID DURING RESPIRATORY ADAPTATION OF YEAST. Biochim Biophys Acta. 1963 Dec 20;76:501–524. [PubMed] [Google Scholar]
  15. Sinclair J. H., Stevens B. J., Sanghavi P., Rabinowitz M. Mitochondrial-satellite and circular DNA filaments in yeast. Science. 1967 Jun 2;156(3779):1234–1237. doi: 10.1126/science.156.3779.1234. [DOI] [PubMed] [Google Scholar]
  16. Slonimski P. P., Perrodin G., Croft J. H. Ethidium bromide induced mutation of yeast mitochondria: complete transformation of cells into respiratory deficient non-chromosomal "petites". Biochem Biophys Res Commun. 1968 Feb 15;30(3):232–239. doi: 10.1016/0006-291x(68)90440-3. [DOI] [PubMed] [Google Scholar]
  17. Smith D., Tauro P., Schweizer E., Halvorson H. O. The replication of mitochondrial DNA during the cell cycle in Saccharomyces lactis. Proc Natl Acad Sci U S A. 1968 Jul;60(3):936–942. doi: 10.1073/pnas.60.3.936. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Sugimura T., Okabe K., Imamura A. Number of cytoplasmic factors in yeast cells. Nature. 1966 Oct 15;212(5059):304–304. doi: 10.1038/212304a0. [DOI] [PubMed] [Google Scholar]
  19. TUSTANOFF E. R., BARTLEY W. THE EFFECT OF GLUCOSE ON THE DEVELOPMENT OF RESPIRATION BY ANAEROBICALLY GROWN YEAST. Can J Biochem. 1964 May;42:651–665. doi: 10.1139/o64-078. [DOI] [PubMed] [Google Scholar]
  20. Tewari K. K., Vötsch W., Mahler H. R., Mackler B. Biochemical correlates of respiratory deficiency. VI. Mitochondrial DNA. J Mol Biol. 1966 Oct;20(3):453–481. doi: 10.1016/0022-2836(66)90003-9. [DOI] [PubMed] [Google Scholar]
  21. Thomas D. Y., Wilkie D. Recombination of mitochondrial drug-resistance factors in Saccharomyces cerevisiae. Biochem Biophys Res Commun. 1968 Feb 26;30(4):368–372. doi: 10.1016/0006-291x(68)90753-5. [DOI] [PubMed] [Google Scholar]
  22. WALLACE P. G., LINNANE A. W. OXYGEN-INDUCED SYNTHESIS OF YEAST MITOCHONDRIA. Nature. 1964 Mar 21;201:1191–1194. doi: 10.1038/2011191a0. [DOI] [PubMed] [Google Scholar]
  23. WILKIE D. THE INDUCTION BY MONOCHROMATIC U.V. LIGHT OF RESPIRATORY-DEFICIENT MUTANTS IN AEROBIC AND ANAEROBIC CULTURES OF YEAST. J Mol Biol. 1963 Nov;7:527–533. doi: 10.1016/s0022-2836(63)80099-6. [DOI] [PubMed] [Google Scholar]
  24. Wallace P. G., Huang M., Linnane A. W. The biogenesis of mitochondria. II. The influence of medium composition on the cytology of anaerobically grown Saccharomyces cerevisiae. J Cell Biol. 1968 May;37(2):207–220. doi: 10.1083/jcb.37.2.207. [DOI] [PMC free article] [PubMed] [Google Scholar]
  25. YOTSUYANAGI Y. [Study of yeast mitochondria. II. Mitochondria of respiration-deficient mutants]. J Ultrastruct Res. 1962 Aug;7:141–158. doi: 10.1016/s0022-5320(62)80032-x. [DOI] [PubMed] [Google Scholar]

Articles from Journal of Bacteriology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES