Skip to main content
NCBI home page
Annals of The Royal College of Surgeons of England logoLink to Annals of The Royal College of Surgeons of England
. 1986 Nov;68(6):302–306.

The localization of anti-tumour monoclonal antibodies in colorectal cancer.

N C Armitage
PMCID: PMC2498300  PMID: 3492956

Abstract

The localization of antitumour monoclonal antibodies was studied in patients with colorectal cancer after intravenous injection. The monoclonal antibody 791T/36 was shown to localise in primary and secondary disease with a preferential uptake of 2.5:1 compared with normal colon. Improved imaging properties were achieved by changing the radiolabel from 131I to 111In and by emission tomography. Using an anti-CEA antibody, C46, a preferential rate of uptake of 5.8:1 was achieved giving superior images and enhancing the prospects of effective targeting of antitumour agents.

Full text

PDF
302

Images in this article

303Fig. 1a
on p.303
303Fig. 1b
on p.303

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Armitage N. C., Perkins A. C., Pimm M. V., Farrands P. A., Baldwin R. W., Hardcastle J. D. The localization of an anti-tumour monoclonal antibody (791T/36) in gastrointestinal tumours. Br J Surg. 1984 Jun;71(6):407–412. doi: 10.1002/bjs.1800710602. [DOI] [PubMed] [Google Scholar]
  2. Armitage N. C., Perkins A. C., Pimm M. V., Wastie M. L., Baldwin R. W., Hardcastle J. D. Imaging of primary and metastatic colorectal cancer using an 111In-labelled antitumour monoclonal antibody (791T/36). Nucl Med Commun. 1985 Oct;6(10):623–631. doi: 10.1097/00006231-198510000-00003. [DOI] [PubMed] [Google Scholar]
  3. Begent R. H., Keep P. A., Green A. J., Searle F., Bagshawe K. D., Jewkes R. F., Jones B. E., Barratt G. M., Ryman B. E. Liposomally entrapped second antibody improves tumour imaging with radiolabelled (first) antitumour antibody. Lancet. 1982 Oct 2;2(8301):739–742. doi: 10.1016/s0140-6736(82)90923-0. [DOI] [PubMed] [Google Scholar]
  4. Begent R. H., Keep P. A., Searle F., Green A. J., Mitchell H. D., Jones B. E., Dent J., Pendower J. E., Parkins R. A., Reynolds K. W. Radioimmunolocalization and selection for surgery in recurrent colorectal cancer. Br J Surg. 1986 Jan;73(1):64–67. doi: 10.1002/bjs.1800730126. [DOI] [PubMed] [Google Scholar]
  5. Berche C., Mach J. P., Lumbroso J. D., Langlais C., Aubry F., Buchegger F., Carrel S., Rougier P., Parmentier C., Tubiana M. Tomoscintigraphy for detecting gastrointestinal and medullary thyroid cancers: first clinical results using radiolabelled monoclonal antibodies against carcinoembryonic antigen. Br Med J (Clin Res Ed) 1982 Nov 20;285(6353):1447–1451. doi: 10.1136/bmj.285.6353.1447. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Buchegger F., Haskell C. M., Schreyer M., Scazziga B. R., Randin S., Carrel S., Mach J. P. Radiolabeled fragments of monoclonal antibodies against carcinoembryonic antigen for localization of human colon carcinoma grafted into nude mice. J Exp Med. 1983 Aug 1;158(2):413–427. doi: 10.1084/jem.158.2.413. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Campbell D. G., Price M. R., Baldwin R. W. Analysis of a human osteogenic sarcoma antigen and its expression on various human tumour cell lines. Int J Cancer. 1984 Jul 15;34(1):31–37. doi: 10.1002/ijc.2910340107. [DOI] [PubMed] [Google Scholar]
  8. Chatal J. F., Saccavini J. C., Fumoleau P., Douillard J. Y., Curtet C., Kremer M., Le Mevel B., Koprowski H. Immunoscintigraphy of colon carcinoma. J Nucl Med. 1984 Mar;25(3):307–314. [PubMed] [Google Scholar]
  9. DeLand F. H., Kim E. E., Simmons G., Goldenberg D. M. Imaging approach in radioimmunodetection. Cancer Res. 1980 Aug;40(8 Pt 2):3046–3049. [PubMed] [Google Scholar]
  10. Dykes P. W., Hine K. R., Bradwell A. R., Blackburn J. C., Reeder T. A., Drolc Z., Booth S. N. Localisation of tumour deposits by external scanning after injection of radiolabelled anti-carcinoembryonic antigen. Br Med J. 1980 Jan 26;280(6209):220–222. doi: 10.1136/bmj.280.6209.220. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Embleton M. J., Gunn B., Byers V. S., Baldwin R. W. Antitumour reactions of monoclonal antibody against a human osteogenic-sarcoma cell line. Br J Cancer. 1981 May;43(5):582–587. doi: 10.1038/bjc.1981.87. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Embleton M. J., Rowland G. F., Simmonds R. G., Jacobs E., Marsden C. H., Baldwin R. W. Selective cytotoxicity against human tumour cells by a vindesine-monoclonal antibody conjugate. Br J Cancer. 1983 Jan;47(1):43–49. doi: 10.1038/bjc.1983.5. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Epenetos A. A., Britton K. E., Mather S., Shepherd J., Granowska M., Taylor-Papadimitriou J., Nimmon C. C., Durbin H., Hawkins L. R., Malpas J. S. Targeting of iodine-123-labelled tumour-associated monoclonal antibodies to ovarian, breast, and gastrointestinal tumours. Lancet. 1982 Nov 6;2(8306):999–1005. doi: 10.1016/s0140-6736(82)90046-0. [DOI] [PubMed] [Google Scholar]
  14. Epenetos A. A., Snook D., Hooker G., Lavender J. P., Halnan K. E. Tumour imaging using an improved method of DTPA-coupled monoclonal antibodies radiolabelled with metallic radionuclides. Lancet. 1984 Jul 21;2(8395):169–169. doi: 10.1016/s0140-6736(84)91087-0. [DOI] [PubMed] [Google Scholar]
  15. FOLEY E. J. Antigenic properties of methylcholanthrene-induced tumors in mice of the strain of origin. Cancer Res. 1953 Dec;13(12):835–837. [PubMed] [Google Scholar]
  16. Farrands P. A., Perkins A. C., Pimm M. V., Embleton M. J., Hardy J. D., Baldwin R. W., Hardcastle J. D. Radioimmunodetection of human colorectal cancers by an anti-tumour monoclonal antibody. Lancet. 1982 Aug 21;2(8295):397–400. doi: 10.1016/s0140-6736(82)90437-8. [DOI] [PubMed] [Google Scholar]
  17. Ford C. H., Newman C. E., Johnson J. R., Woodhouse C. S., Reeder T. A., Rowland G. F., Simmonds R. G. Localisation and toxicity study of a vindesine-anti-CEA conjugate in patients with advanced cancer. Br J Cancer. 1983 Jan;47(1):35–42. doi: 10.1038/bjc.1983.4. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Fraker P. J., Speck J. C., Jr Protein and cell membrane iodinations with a sparingly soluble chloroamide, 1,3,4,6-tetrachloro-3a,6a-diphrenylglycoluril. Biochem Biophys Res Commun. 1978 Feb 28;80(4):849–857. doi: 10.1016/0006-291x(78)91322-0. [DOI] [PubMed] [Google Scholar]
  19. Gilliland D. G., Steplewski Z., Collier R. J., Mitchell K. F., Chang T. H., Koprowski H. Antibody-directed cytotoxic agents: use of monoclonal antibody to direct the action of toxin A chains to colorectal carcinoma cells. Proc Natl Acad Sci U S A. 1980 Aug;77(8):4539–4543. doi: 10.1073/pnas.77.8.4539. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Goldenberg D. M., DeLand F., Kim E., Bennett S., Primus F. J., van Nagell J. R., Jr, Estes N., DeSimone P., Rayburn P. Use of radiolabeled antibodies to carcinoembryonic antigen for the detection and localization of diverse cancers by external photoscanning. N Engl J Med. 1978 Jun 22;298(25):1384–1386. doi: 10.1056/NEJM197806222982503. [DOI] [PubMed] [Google Scholar]
  21. KORNGOLD L., PRESSMAN D. The localization of antilymphosarcoma antibodies in the Murphy lymphosarcoma of the rat. Cancer Res. 1954 Feb;14(2):96–99. [PubMed] [Google Scholar]
  22. Köhler G., Milstein C. Continuous cultures of fused cells secreting antibody of predefined specificity. Nature. 1975 Aug 7;256(5517):495–497. doi: 10.1038/256495a0. [DOI] [PubMed] [Google Scholar]
  23. Mach J. P., Chatal J. F., Lumbroso J. D., Buchegger F., Forni M., Ritschard J., Berche C., Douillard J. Y., Carrel S., Herlyn M. Tumor localization in patients by radiolabeled monoclonal antibodies against colon carcinoma. Cancer Res. 1983 Nov;43(11):5593–5600. [PubMed] [Google Scholar]
  24. Moldofsky P. J., Powe J., Mulhern C. B., Jr, Hammond N., Sears H. F., Gatenby R. A., Steplewski Z., Koprowski H. Metastatic colon carcinoma detected with radiolabeled F(ab')2 monoclonal antibody fragments. Radiology. 1983 Nov;149(2):549–555. doi: 10.1148/radiology.149.2.6622704. [DOI] [PubMed] [Google Scholar]
  25. Perkins A. C., Pimm M. V., Birch M. K. The preparation and characterisation of 111In-labelled 791T/36 monoclonal antibody for tumour immunoscintigraphy. Eur J Nucl Med. 1985;10(7-8):296–301. doi: 10.1007/BF00251299. [DOI] [PubMed] [Google Scholar]
  26. Pimm M. V., Perkins A. C., Armitage N. C., Baldwin R. W. The characteristics of blood-borne radiolabels and the effect of anti-mouse IgG antibodies on localization of radiolabeled monoclonal antibody in cancer patients. J Nucl Med. 1985 Sep;26(9):1011–1023. [PubMed] [Google Scholar]
  27. Price M. R., Campbell D. G., Robins R. A., Baldwin R. W. Characteristics of a cell surface antigen defined by an anti-human osteogenic sarcoma monoclonal antibody. Eur J Cancer Clin Oncol. 1983 Jan;19(1):81–90. doi: 10.1016/0277-5379(83)90402-9. [DOI] [PubMed] [Google Scholar]
  28. Price M. R., Pimm M. V., Page C. M., Armitage N. C., Hardcastle J. D., Baldwin R. W. Immunolocalization of the murine monoclonal antibody, 791T/36 within primary human colorectal carcinomas and identification of the target antigen. Br J Cancer. 1984 Jun;49(6):809–812. doi: 10.1038/bjc.1984.126. [DOI] [PMC free article] [PubMed] [Google Scholar]
  29. Rainsbury R. M. The localization of human breast carcinomas by radiolabelled monoclonal antibodies. Br J Surg. 1984 Oct;71(10):805–812. doi: 10.1002/bjs.1800711023. [DOI] [PubMed] [Google Scholar]
  30. Smedley H. M., Finan P., Lennox E. S., Ritson A., Takei F., Wraight P., Sikora K. Localisation of metastatic carcinoma by a radiolabelled monoclonal antibody. Br J Cancer. 1983 Feb;47(2):253–259. doi: 10.1038/bjc.1983.33. [DOI] [PMC free article] [PubMed] [Google Scholar]
  31. Wahl R. L., Parker C. W., Philpott G. W. Improved radioimaging and tumor localization with monoclonal F(ab')2. J Nucl Med. 1983 Apr;24(4):316–325. [PubMed] [Google Scholar]

Articles from Annals of The Royal College of Surgeons of England are provided here courtesy of The Royal College of Surgeons of England

RESOURCES