Skip to main content
NCBI home page
Journal of Bacteriology logoLink to Journal of Bacteriology
. 1969 Mar;97(3):1321–1327. doi: 10.1128/jb.97.3.1321-1327.1969

Phosphatidic Acid Synthesis in Escherichia coli

Makoto Kito a,1, Lewis I Pizer a
PMCID: PMC249850  PMID: 4887512

Abstract

The kinetic properties of acyl-coenzyme A (CoA): l-α-glycerol-phosphate trans-acylase (EC 2.3.1.15) from Escherichia coli were studied. At 10 C, a temperature at which the reaction was proportional to time and enzyme concentration, the enzyme had an apparent Km of 60 μm for l-α-glycerol-phosphate. The curve describing the velocity of the reaction as a function of palmitoyl-CoA concentration was sigmoid but the plot of v−1 versus [S]−3 gave a straight line. A Km of about 11 μm was calculated for palmitoyl-CoA. Adenosine triphosphate specifically inhibited the reaction, being a noncompetitive inhibitor in respect to l-α-glycerol phosphate. Inhibition only occurred with high concentrations of palmitoyl-CoA, and maximal inhibition was 60%.

Full text

PDF
1321

Images in this article

1324Image
on p.1324

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Ailhaud G. P., Vagelos P. R. Palmityl-acyl carrier protein as acyl donor for complex lipid biosynthesis in Escherichia coli. J Biol Chem. 1966 Aug 25;241(16):3866–3869. [PubMed] [Google Scholar]
  2. BALLOU C. E., VILKAS E., LEDERER E. Structural studies on the myo-inositol phospholipids of Mycobacterium tuberculosis (var. bovis, strain BCG). J Biol Chem. 1963 Jan;238:69–76. [PubMed] [Google Scholar]
  3. BANDURSKI R. S., AXELROD B. The chromatographic identification of some biologically important phosphate esters. J Biol Chem. 1951 Nov;193(1):405–410. [PubMed] [Google Scholar]
  4. BUBLITZ C., KENNEDY E. P. Synthesis of phosphatides in isolated mitochondria. III. The enzymatic phosphorylation of glycerol. J Biol Chem. 1954 Dec;211(2):951–961. [PubMed] [Google Scholar]
  5. Chang Y. Y., Kennedy E. P. Pathways for the synthesis of glycerophosphatides in Escherichia coli. J Biol Chem. 1967 Feb 10;242(3):516–519. [PubMed] [Google Scholar]
  6. Furrow M. H., Pizer L. I. Phospholipid synthesis in Escherichia coli infected with T4 bacteriophages. J Virol. 1968 Jun;2(6):594–605. doi: 10.1128/jvi.2.6.594-605.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. GOLDSTEIN D. B., BROWN B. J., GOLDSTEIN A. Amino acid starvation in an Escherichia coli auxotroph. II. Acidsoluble purine and pyrimidine derivatives in normal and starved cells. Biochim Biophys Acta. 1960 Sep 9;43:55–61. doi: 10.1016/0006-3002(60)90406-6. [DOI] [PubMed] [Google Scholar]
  8. Goldfine H. Use of a filter-paper disk assay in the measurement of lipid biosynthesis. J Lipid Res. 1966 Jan;7(1):146–149. [PubMed] [Google Scholar]
  9. JATZKEWITZ H., MEHL E. [On thin layer chromatography of cerebral lipids, their conversion and decomposition products]. Hoppe Seylers Z Physiol Chem. 1960 Oct 17;320:251–257. doi: 10.1515/bchm2.1960.320.1.251. [DOI] [PubMed] [Google Scholar]
  10. Kito M., Pizer L. I. The biosynthetic L-alpha-glycerol phosphate dehydrogenase of E. coli. Biochem Biophys Res Commun. 1968 Aug 13;32(3):408–412. doi: 10.1016/0006-291x(68)90676-1. [DOI] [PubMed] [Google Scholar]
  11. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  12. Lubochinsky B., Meury J., Stolkowski J. Transport du potassium et synthèse des phospholipides chez l'Escherichia coli. I. Les phospholipides des souches B 163 et B 525. Bull Soc Chim Biol (Paris) 1965;47(7):1529–1532. [PubMed] [Google Scholar]
  13. MONOD J., CHANGEUX J. P., JACOB F. Allosteric proteins and cellular control systems. J Mol Biol. 1963 Apr;6:306–329. doi: 10.1016/s0022-2836(63)80091-1. [DOI] [PubMed] [Google Scholar]
  14. PIERINGER R. A., KUNNES R. S. THE BIOSYNTHESIS OF PHOSPHATIDIC ACID AND LYSOPHOSPHATIDIC ACID BY GLYCERIDE PHOSPHOKINASE PATHWAYS IN ESCHERICHIA COLI. J Biol Chem. 1965 Jul;240:2833–2838. [PubMed] [Google Scholar]
  15. PIZER L. I., POTOCHNY M. L. NUTRITIONAL AND REGULATORY ASPECTS OF SERINE METABOLISM IN ESCHERICHIA COLI. J Bacteriol. 1964 Sep;88:611–619. doi: 10.1128/jb.88.3.611-619.1964. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Pieringer R. A., Bonner H., Jr, Kunnes R. S. Biosynthesis of phosphatidic acid, lysophosphatidic acid, diglyceride, and triglyceride by fatty acyltransferase pathways in Escherichia coli. J Biol Chem. 1967 Jun 10;242(11):2719–2724. [PubMed] [Google Scholar]
  17. TARLOV A. R., KENNEDY E. P. THE BETA-GALACTOSIDE PERMEASE SYSTEM AND THE METABOLISM OF PHOSPHOLIPIDS IN ESCHERICHIA COLI. J Biol Chem. 1965 Jan;240:49–53. [PubMed] [Google Scholar]
  18. Taketa K., Pogell B. M. The effect of palmityl coenzyme A on glucose 6-phosphate dehydrogenase and other enzymes. J Biol Chem. 1966 Feb 10;241(3):720–726. [PubMed] [Google Scholar]
  19. UMBARGER H. E. Feedback control by endproduct inhibition. Cold Spring Harb Symp Quant Biol. 1961;26:301–312. doi: 10.1101/sqb.1961.026.01.036. [DOI] [PubMed] [Google Scholar]

Articles from Journal of Bacteriology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES