Abstract
A method has been developed for growing Physarum polycephalum plasmodia that are 8 to 10 times larger than those obtained in the petri dish cultures used by Nygaard, Guttes, and Rusch. In the large-scale procedure, plasmodia were grown in metal trays on a membrane supported by filter paper on stainless-steel screen. Plasmodia were started from a ring of inoculum to allow inward and outward migration and were incubated on a rocker so that nutrient medium would flow back and forth, wetting the undersurface of the plasmodium. Rocker and petri dish cultures had similar growth characteristics: (i) the interphase time between mitoses I and II and between II and III was about 8 hr; (ii) ribonucleic acid and protein increased essentially logarithmically throughout the cell cycle; and (iii) deoxyribonucleic acid increased only during early interphase and it doubled in approximately 3 hr after each mitosis. Rocker cultures were not as nearly synchronous as petri dish cultures and had a range in metaphase time (at mitosis III) within individual plasmodia of 15 to 45 min, as compared with 5 to 10 min in petri dish cultures.
Full text
PDF
Images in this article
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Cummins J. E., Blomquist J. C., Rusch H. P. Anaphase delay after inhibition of protein synthesis between late prophase and prometaphase. Science. 1966 Dec 9;154(3754):1343–1344. doi: 10.1126/science.154.3754.1343. [DOI] [PubMed] [Google Scholar]
- GUTTES E., GUTTES S., RUSCH H. P. Morphological observations on growth and differentation of Physarum polycephalum grown in pure culture. Dev Biol. 1961 Oct;3:588–614. doi: 10.1016/0012-1606(61)90034-3. [DOI] [PubMed] [Google Scholar]
- Guttes S., Guttes E., Ellis R. A. Electron microscope study of mitosis in Physarum polycephalum. J Ultrastruct Res. 1968 Mar;22(5):508–529. doi: 10.1016/s0022-5320(68)90038-5. [DOI] [PubMed] [Google Scholar]
- LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
- MITTERMAYER C., BRAUN R., RUSCH H. P. THE EFFECT OF ACTINOMYCIN D ON THE TIMING OF MITOSIS IN PHYSARUM POLYCEPHALUM. Exp Cell Res. 1965 Apr;38:33–41. doi: 10.1016/0014-4827(65)90424-6. [DOI] [PubMed] [Google Scholar]
- Mittermayer C., Braun R., Chayka T. G., Rusch H. P. Polysome patterns and protein synthesis during the mitotic cycle of Physarum polycephalum. Nature. 1966 Jun 11;210(5041):1133–1137. doi: 10.1038/2101133a0. [DOI] [PubMed] [Google Scholar]
- NYGAARD O. F., GUTTES S., RUSCH H. P. Nucleic acid metabolism in a slime mold with synchronous mitosis. Biochim Biophys Acta. 1960 Feb 26;38:298–306. doi: 10.1016/0006-3002(60)91245-2. [DOI] [PubMed] [Google Scholar]
- SACHSENMAIER W., RUSCH H. P. THE EFFECT OF 5-FLUORO-2'-DEOXYURIDINE ON SYNCHRONOUS MITOSIS IN PHYSARUM POLYCEPHALUM. Exp Cell Res. 1964 Oct;36:124–133. doi: 10.1016/0014-4827(64)90166-1. [DOI] [PubMed] [Google Scholar]