Abstract
The principal phospholipids of Bacillus megaterium throughout the cycle of growth and sporulation were found to be phosphatidylglycerol, diphosphatidylglycerol, phosphatidylethanolamine, and a hitherto unidentified isomer of glycosaminyl-phosphatidylglycerol. Phosphatidylglycerol predominated during vegetative cell growth and then declined as spores developed, whereas diphosphatidylglycerol became more prominent during spore maturation. The latter phosphatide was relatively inaccessible in the vegetative cell and was more accessible in the spore, as judged by solvent extraction under various conditions.
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- BLIGH E. G., DYER W. J. A rapid method of total lipid extraction and purification. Can J Biochem Physiol. 1959 Aug;37(8):911–917. doi: 10.1139/o59-099. [DOI] [PubMed] [Google Scholar]
- Bonsen P. P., de Haas G. H., van Deenen L. L. Synthesis and enzymic hydrolysis of an O-alanyl ester of phosphatidyl glycerol. Biochim Biophys Acta. 1965 Jul 7;106(1):93–105. doi: 10.1016/0005-2760(65)90098-6. [DOI] [PubMed] [Google Scholar]
- Bonsen P. P., de Haas G. H., van Deenen L. L. Synthetic and structural investigations on 3-phosphatidyl-1'-(3'-O-L-lysyl)glycerol. Biochemistry. 1967 Apr;6(4):1114–1120. doi: 10.1021/bi00856a021. [DOI] [PubMed] [Google Scholar]
- CRESTFIELD A. M., STEIN W. H., MOORE S. On the preparation of bovine pancreatic ribonuclease A. J Biol Chem. 1963 Feb;238:618–621. [PubMed] [Google Scholar]
- DAWSON R. M. A hydrolytic procedure for the identification and estimation of individual phospholipids in biological samples. Biochem J. 1960 Apr;75:45–53. doi: 10.1042/bj0750045. [DOI] [PMC free article] [PubMed] [Google Scholar]
- DAWSON R. M., HEMINGTON N., DAVENPORT J. B. Improvements in the method of determining individual phospholipids in a complex mixture by successive chemical hydrolyses. Biochem J. 1962 Sep;84:497–501. doi: 10.1042/bj0840497. [DOI] [PMC free article] [PubMed] [Google Scholar]
- FITZ-JAMES P. C. The phosphorus fractions of Bacillus cereus and Bacillus megaterium. I. A comparison of spores and vegetative cells. Can J Microbiol. 1955 Aug;1(7):502–519. doi: 10.1139/m55-064. [DOI] [PubMed] [Google Scholar]
- Fleischer S., Fleischer B., Stoeckenius W. Fine structure of lipid-depleted mitochondria. J Cell Biol. 1967 Jan;32(1):193–208. doi: 10.1083/jcb.32.1.193. [DOI] [PMC free article] [PubMed] [Google Scholar]
- GERHARDT P., RIBI E. ULTRASTRUCTURE OF THE EXOSPORIUM ENVELOPING SPORES OF BACILLUS CEREUS. J Bacteriol. 1964 Dec;88:1774–1789. doi: 10.1128/jb.88.6.1774-1789.1964. [DOI] [PMC free article] [PubMed] [Google Scholar]
- HANES C. S., ISHERWOOD F. A. Separation of the phosphoric esters on the filter paper chromatogram. Nature. 1949 Dec 31;164(4183):1107-12, illust. doi: 10.1038/1641107a0. [DOI] [PubMed] [Google Scholar]
- Kates M. Bacterial lipids. Adv Lipid Res. 1964;2:17–90. [PubMed] [Google Scholar]
- Lennarz W. J., Bonsen P. P., van Deenen L. L. Substrate specificity of O-L-lysylphosphatidylglycerol synthetase. Enzymatic studies on the structure of O-L-lysylphosphatidylglycerol. Biochemistry. 1967 Aug;6(8):2307–2312. doi: 10.1021/bi00860a005. [DOI] [PubMed] [Google Scholar]
- MARINETTI G. V., ERBLAND J., STOTZ E. Phosphatides of pig heart cell fractions. J Biol Chem. 1958 Sep;233(3):562–565. [PubMed] [Google Scholar]
- MATCHES J. R., WALKER H. W., AYRES J. C. PHOSPHOLIPIDS IN VEGETATIVE CELLS AND SPORES OF BACILLUS POLYMYXA. J Bacteriol. 1964 Jan;87:16–23. doi: 10.1128/jb.87.1.16-23.1964. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Phizackerley P. J., MacDougall J. C., Francis M. J. Phosphatidylglycerylglucosamine. Biochem J. 1966 Jun;99(3):21C–22C. doi: 10.1042/bj0990021c. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Scandella C. J., Kornberg A. Biochemical studies of bacterial sporulation and germination. XV. Fatty acids in growth, sporulation, and germination of Bacillus megaterium. J Bacteriol. 1969 Apr;98(1):82–86. doi: 10.1128/jb.98.1.82-86.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
- den Kamp JA O. P., Houtsmuller U. M., van Deenen L. L. On the phospholipids of Bacillus megaterium. Biochim Biophys Acta. 1965 Oct 4;106(2):438–441. doi: 10.1016/0005-2760(65)90059-7. [DOI] [PubMed] [Google Scholar]
- den Kamp JA O. P., van Iterson W., van Deenen L. L. Studies of the phospholipids and morphology of protoplasts of Bacillus megaterium. Biochim Biophys Acta. 1967;135(5):862–884. doi: 10.1016/0005-2736(67)90056-9. [DOI] [PubMed] [Google Scholar]