Abstract
The segregation of mating loci with other unselected genes was analyzed in recombinants obtained from matings of Nocardia canicruria and N. erythropolis. The loci C/c and E/e control nocardial compatibility. Four mating genotype combinations were observed: cE, Ce, CE, and ce. Strains of N. erythropolis bear the genotype cE and strains of N. canicruria bear the Ce alleles. The CE recombinant mating type is capable of mating with both organisms, whereas the ce-containing recombinant is nonfertile. The E locus was found to segregate with StrA1 (streptomycin-resistance gene) on the N. erythropolis linkage group. The C locus appeared linked to PurB2 (purine-requiring gene) on the N. canicruria linkage group. A few observed recombinants were capable of further segregation of unselected characters after colonial purification, suggesting a possible heterogenomic condition or multiple rounds of mating. Prior treatment of recombinants with acriflavine failed to alter their compatibility or the frequency at which recombinants were recovered. The segregation pattern of the mating loci allowed for specific recombinant class types to be compatible.
Full text
PDF
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- ADAMS J. N. RECOMBINATION BETWEEN NOCARDIA ERYTHROPOLIS AND NOCARDIA CANICRURIA. J Bacteriol. 1964 Oct;88:865–876. doi: 10.1128/jb.88.4.865-876.1964. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Adams J. N., Bradley S. G. Recombination Events in the Bacterial Genus Nocardia. Science. 1963 Jun 28;140(3574):1392–1394. doi: 10.1126/science.140.3574.1392. [DOI] [PubMed] [Google Scholar]
- Adams J. N. Partial exclusion of the Nocardia erythropolis chromosome in nocardial recombinants. J Bacteriol. 1968 Nov;96(5):1750–1759. doi: 10.1128/jb.96.5.1750-1759.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Brownell G. H., Adams J. N., Bradley S. G. Growth and characterization of nocardiophages for Nocardia canicruria and Nocardia erythropolis mating types. J Gen Microbiol. 1967 May;47(2):247–256. doi: 10.1099/00221287-47-2-247. [DOI] [PubMed] [Google Scholar]
- Brownell G. H., Adams J. N. Linkage and segregation of a mating type specific phage and resistance characters in nocardial recombinants. Genetics. 1968 Nov;60(3):437–448. doi: 10.1093/genetics/60.3.437. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Brownell G. H., Adams J. N. Linkage and segregation of unselected markers in matings of Nocardia erythropolis with Nocardia canicruria. J Bacteriol. 1967 Sep;94(3):650–659. doi: 10.1128/jb.94.3.650-659.1967. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Demerec M., Adelberg E. A., Clark A. J., Hartman P. E. A proposal for a uniform nomenclature in bacterial genetics. Genetics. 1966 Jul;54(1):61–76. doi: 10.1093/genetics/54.1.61. [DOI] [PMC free article] [PubMed] [Google Scholar]