Skip to main content
Journal of Bacteriology logoLink to Journal of Bacteriology
. 1969 Jul;99(1):132–135. doi: 10.1128/jb.99.1.132-135.1969

Antigenic Structure of Treponema pallidum, Nichols Strain II. Extraction of a Polysaccharide Antigen with “Strain-specific” Serological Activity

James N Miller 1,2, J H Bekker 1,2, J H DeBruijn 1,2, P C Onvlee 1,2
PMCID: PMC249977  PMID: 4895841

Abstract

An ultracentrifugally homogeneous heat-stable polysaccharide preparation free from serologically reactive rabbit testicular tissue antigen, including cardiolipin, was extracted from the Nichols strain of Treponema pallidum, and found to react by complement-fixation with homologous rabbit sera but not with human syphilitic sera. In addition, the reactive “strain-specific” component was found to be distinct from a second reactive component within the preparation related to an antigen of T. reiteri.

Full text

PDF

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. BEKKER J. H. Specificity and sensitivity of the Reiter protein complement-fixation (RPCF) test. Report on 1,400 selected sera. Br J Vener Dis. 1959 Jun;35:129–131. doi: 10.1136/sti.35.2.129. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Bekker J. H., de Bruijn J. H., Miller J. N. Performance and use of the Reiter protein complement-fixation (RPCF) test. Br J Vener Dis. 1966 Mar;42(1):42–43. doi: 10.1136/sti.42.1.42. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. DE BRUIJN J. H. A simple method to absorb non-precipitating antibodies from immune serum. Antonie Van Leeuwenhoek. 1956;22(4):350–352. doi: 10.1007/BF02538348. [DOI] [PubMed] [Google Scholar]
  4. HARDY P. H., Jr, NELL E. E. Specific agglutination of Treponema pallidum by sera from rabbits and human beings with treponemal infections. J Exp Med. 1955 Apr 1;101(4):367–382. doi: 10.1084/jem.101.4.367. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. JULIAN A. J., PORTNOY J., BOSSAK H. N. False positive reactions in treponemal tests. Br J Vener Dis. 1963 Mar;39:30–32. doi: 10.1136/sti.39.1.30. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. KAPUSCINSKI V., ZAK B. Use of perchloric acid filtrate and stabilized anthrone for determination of serum glucose. Am J Clin Pathol. 1953 Aug;23(8):784–788. doi: 10.1093/ajcp/23.8.784. [DOI] [PubMed] [Google Scholar]
  7. KENT J. F., DE WEERDT J. B. Enhancement by lysozyme of the sensitivity of Treponema pallidum immobilization tests. Br J Vener Dis. 1963 Mar;39:37–40. doi: 10.1136/sti.39.1.37. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. MANSI W. Slide gel diffusion precipitin test. Nature. 1958 May 3;181(4618):1289–1290. doi: 10.1038/1811289b0. [DOI] [PubMed] [Google Scholar]
  9. MILLER J. N. IMMUNITY IN EXPERIMENTAL SYPHILIS. 3. ATTENUATION OF VIRULENT TREPONEMA PALLIDIUM BY GAMMA-IRRADIATION. J Bacteriol. 1965 Aug;90:297–301. doi: 10.1128/jb.90.2.297-301.1965. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. McLeod Charlotte P. Relationship between TPCF antibody and reagin. Public Health Rep. 1962 May;77(5):441–445. [PMC free article] [PubMed] [Google Scholar]
  11. Miller J. N., Bekker J. H., DeBruijn J. H., Onvlee P. C. The immunologic response of goats to normal and syphilitic rabbit testicular tissue. J Immunol. 1966 Aug;97(2):184–188. [PubMed] [Google Scholar]
  12. Miller J. N. Immunity in experimental syphilis. V. The immunogenicity of Treponema pallidum attenuated by gamma-irradiation. J Immunol. 1967 Nov;99(5):1012–1016. [PubMed] [Google Scholar]
  13. Miller J. N., de Bruijn J. H., Bekker J. H. Immunity in experimental syphilis. IV. Serological reactivity of antigens extracted from gamm-irradiated Treponema pallidum and Treponema reiteri. J Bacteriol. 1966 Feb;91(2):583–587. doi: 10.1128/jb.91.2.583-587.1966. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Miller J. N., de Bruijn J. H., Bekker J. H., Onvlee P. C. The antigenic structure of Treponema pallidum, Nichols strain. I. The demonstration, nature and location of specific and shared antigens. J Immunol. 1966 Mar;96(3):450–456. [PubMed] [Google Scholar]
  15. STUDIES ON THE ANTIGENIC STRUCTURE OF T. PALLIDUM. 5. ATTEMPTS TO ISOLATE POLYSACCHARIDE ANTIGEN FROM NICHOLS' PATHOGENIC STRAIN. Acta Pathol Microbiol Scand. 1964;61:141–149. doi: 10.1111/apm.1964.61.1.141. [DOI] [PubMed] [Google Scholar]

Articles from Journal of Bacteriology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES