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. 1969 Jul;99(1):197–205. doi: 10.1128/jb.99.1.197-205.1969

Tricarboxylic Acid Cycle Enzymes and Morphogenesis in Blastocladiella emersonii

Boen Tie Khouw 1, Howard D McCurdy 2
PMCID: PMC249987  PMID: 5802605

Abstract

During exponential growth, ordinary colorless (OC) plants of Blastocladiella emersonii consumed little glucose and produced no lactic acid. Similarly, resistant sporangial (RS) plants did not utilize glucose or produce lactic acid during the first 24 hr of exponential growth. During the next 24 hr of RS development, glucose was consumed with the concomitant production of lactic acid which was then reutilized. Lactic acid gradually accumulated again at maturity. Enzyme studies on cell-free extracts indicated the presence of all tricarboxylic cycle enzymes except α-ketoglutarate dehydrogenase at all stages of development of both RS and OC plants. Included among the enzymes detected were an adenosine monophosphate-stimulated, nicotinamide adenine dinucleotide-isocitric dehydrogenase, and citrate-condensing enzyme. When measured on a per plant basis, tricarboxylic cycle enzyme levels increased during the exponential growth of both kinds of plants. Only after the bicarbonate ceased to have effect on RS plant morphogenesis was there a decrease in the levels of the tricarboxylic cycle enzymes when measured on a per plant basis. Specific activity measurements indicated some differences in the differential rates of synthesis among the enzymes studied previous to 36 hr. Preliminary studies utilizing short periods of 14C-bicarbonate fixation in young RS plants indicated that during the first 4 min most of the label was located in aspartic acid. These results are discussed in terms of previous results and particularly Cantino's hypothesis concerning the relationship between bicarbonate induction and tricarboxylic-cycle enzymes in the morphogenesis of B. emersonii.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. CANTINO E. C., GOLDSTEIN A. Bicarbonate-induced synthesis of polysaccharide during morphogenesis by synchronous, single-generations of blastocladiella emersonii. Arch Mikrobiol. 1961;39:43–52. doi: 10.1007/BF00406526. [DOI] [PubMed] [Google Scholar]
  2. CANTINO E. C., HYATT M. T. Further evidence for the role of the tricarboxylic acid cycle in morphogenesis in Blastocladiella emersonii. J Bacteriol. 1953 Dec;66(6):712–720. doi: 10.1128/jb.66.6.712-720.1953. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. CANTINO E. C., TURIAN G. A role for glycine in light stimulated nucleic acid synthesis by Blastocladiella emersonii. Arch Mikrobiol. 1961;38:272–282. doi: 10.1007/BF00422359. [DOI] [PubMed] [Google Scholar]
  4. ELLS H. A. A colorimetric method for the assay of soluble succinic dehydrogenase and pyridinenucleotide-linked dehydrogenases. Arch Biochem Biophys. 1959 Dec;85:561–562. doi: 10.1016/0003-9861(59)90527-2. [DOI] [PubMed] [Google Scholar]
  5. Griffin D. H. The interaction of hydrogen ion, carbon dioxide and potassium ion in controlling the formation of resistant sporangia in Blastocladiella emersonii. J Gen Microbiol. 1965 Jul;40(1):13–28. doi: 10.1099/00221287-40-1-13. [DOI] [PubMed] [Google Scholar]
  6. HAGER L. P., KORNBERG H. L. On the mechanism of alpha-oxoglutarate oxidation in Escherichia coli. Biochem J. 1961 Jan;78:194–198. doi: 10.1042/bj0780194. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. LOVETT J. S., CANTINO E. C. Reversible bicarbonate-induced enzyme activity and the point of no return during morphogenesis in Blastocladiella. J Gen Microbiol. 1961 Jan;24:87–93. doi: 10.1099/00221287-24-1-87. [DOI] [PubMed] [Google Scholar]
  8. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  9. McCurdy H. D., Cantino E. C. Isocitritase, Glycine-Alanine Transaminase, and Development in Blastocladiella Emersonii. Plant Physiol. 1960 Jul;35(4):463–476. doi: 10.1104/pp.35.4.463. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. McFadden B. A., Kuehn G. D., Homann H. R. CO(2) Fixation, Glutamate Labeling, and the Krebs Cycle in Ribose-grown Hydrogenomonas facilis. J Bacteriol. 1967 Mar;93(3):879–885. doi: 10.1128/jb.93.3.879-885.1967. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. SANADI D. R., LITTLEFIELD J. W. Studies on alpha-ketoglutaric oxidase. I. Formation of "active" succinate. J Biol Chem. 1951 Dec;193(2):683–689. [PubMed] [Google Scholar]
  12. SANWAL B. D., ZINK M. W., STACHOW C. S. CONTROL OF DPN-SPECIFIC ISOCITRIC DEHYDROGENASE ACTIVITY BY PRECURSOR ACTIVATION AND END PRODUCT INHIBITION. Biochem Biophys Res Commun. 1963 Aug 20;12:510–515. doi: 10.1016/0006-291x(63)90325-5. [DOI] [PubMed] [Google Scholar]
  13. SRERE P. A., KOSICKI G. W. The purification of citrate-condensing enzyme. J Biol Chem. 1961 Oct;236:2557–2559. [PubMed] [Google Scholar]
  14. Von Tigerstrom M., Campbell J. J. The accumulation of alpha-ketoglutarate by suspensions of Pseudomonas aeruginosa. Can J Microbiol. 1966 Oct;12(5):1005–1013. doi: 10.1139/m66-135. [DOI] [PubMed] [Google Scholar]
  15. Wood H. G., Utter M. F. The role of CO2 fixation in metabolism. Essays Biochem. 1965;1:1–27. [PubMed] [Google Scholar]

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