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. 1969 Jul;99(1):248–254. doi: 10.1128/jb.99.1.248-254.1969

Characterization of the Deoxyribonucleic Acid of Various Strains of Halophilic Bacteria

Richard L Moore a,1, Brian J McCarthy a
PMCID: PMC249995  PMID: 4979441

Abstract

Bacteria classified as extreme halophiles, in the genera Halobacterium and Halococcus, contain deoxyribonucleic acid (DNA) which displays two components in a CsCl equilibrium density gradient. The base composition of the major DNA component ranges from 66 to 68% guanine plus cytosine (GC), whereas that of the satellite DNA comprising some 11 to 36% of the total, is between 57 and 60% GC. Purification of the bacterial cells in a CsCl density gradient and other more conventional strain purification procedures both indicated that the presence of the satellite DNA component is not a result of mixed cultures.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. ABRAM D., GIBBONS N. E. The effect of chlorides of monovalent cations, urea, detergents, and heat on morphology and the turbidity of suspensions of red halophilic bacteria. Can J Microbiol. 1961 Oct;7:741–750. doi: 10.1139/m61-088. [DOI] [PubMed] [Google Scholar]
  2. ABRAM D., GIBBONS N. E. Turbidity of suspensions and morphology of red halophilic bacteria as influenced by sodium chloride concentration. Can J Microbiol. 1960 Oct;6:535–543. doi: 10.1139/m60-062. [DOI] [PubMed] [Google Scholar]
  3. BAXTER R. M., GIBBONS N. E. Effects of sodium and potassium chloride on certain enzymes of Micrococcus halodenitrificans and Pseudomonas salinaria. Can J Microbiol. 1956 Oct;2(6):599–606. doi: 10.1139/m56-072. [DOI] [PubMed] [Google Scholar]
  4. BAXTER R. M., GIBBONS N. E. The cysteine desulphydrase of Pseudomonas salinaria. Can J Microbiol. 1957 Apr;3(3):461–465. doi: 10.1139/m57-049. [DOI] [PubMed] [Google Scholar]
  5. BAXTER R. M., GIBBONS N. E. The glycerol dehydrogenases of Pseudomonas salinaria, Vibrio costicolus, and Escherichia coli in relation to bacterial halophilism. Can J Biochem Physiol. 1954 May;32(3):206–217. [PubMed] [Google Scholar]
  6. BAYLEY S. T., KUSHNER D. J. THE RIBOSOMES OF THE EXTREMELY HALOPHILIC BACTERIUM, HALOBACTERIUM CUTIRUBRUM. J Mol Biol. 1964 Sep;9:654–669. doi: 10.1016/s0022-2836(64)80173-x. [DOI] [PubMed] [Google Scholar]
  7. BROWN H. J., GIBBONS N. E. The effect of magnesium, potassium, and iron on the growth and morphology of red halophilic bacteria. Can J Microbiol. 1955 Aug;1(7):486–494. doi: 10.1139/m55-062. [DOI] [PubMed] [Google Scholar]
  8. Bayley S. T., Griffiths E. A cell-free amino acid incorporating system from an extremely halophilic bacterium. Biochemistry. 1968 Jun;7(6):2249–2256. doi: 10.1021/bi00846a030. [DOI] [PubMed] [Google Scholar]
  9. Britten R. J., Roberts R. B. High-Resolution Density Gradient Sedimentation Analysis. Science. 1960 Jan 1;131(3392):32–33. doi: 10.1126/science.131.3392.32. [DOI] [PubMed] [Google Scholar]
  10. CHRISTIAN J. H., INGRAM M. The freezing points of bacterial cells in relation to halophilism. J Gen Microbiol. 1959 Feb;20(1):27–31. doi: 10.1099/00221287-20-1-27. [DOI] [PubMed] [Google Scholar]
  11. CHRISTIAN J. H., WALTHO J. A. Solute concentrations within cells of halophilic and non-halophilic bacteria. Biochim Biophys Acta. 1962 Dec 17;65:506–508. doi: 10.1016/0006-3002(62)90453-5. [DOI] [PubMed] [Google Scholar]
  12. Cahn F. H., Fox M. S. Fractionation of transformable bacteria from ocompetent cultures of Bacillus subtilis on renografin gradients. J Bacteriol. 1968 Mar;95(3):867–875. doi: 10.1128/jb.95.3.867-875.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Duchow E., Douglas H. C. RHODOMICROBIUM VANNIELII, A NEW PHOTOHETEROTROPHIC BACTERIUM. J Bacteriol. 1949 Oct;58(4):409–416. doi: 10.1128/jb.58.4.409-416.1949. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Edelman M., Swinton D., Schiff J. A., Epstein H. T., Zeldin B. Deoxyribonucleic Acid of the blue-green algae (cyanophyta). Bacteriol Rev. 1967 Dec;31(4):315–331. doi: 10.1128/br.31.4.315-331.1967. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. HIGUCHI M., GOTO K., FUJIMOTO M., NAMIKI O., KIKUCHI G. EFFECT OF INHIBITORS OF NUCLEIC ACID AND PROTEIN SYNTHESES ON THE INDUCED SYNTHESES OF BACTERIOCHLOROPHYLL AND DELTA-AMINOLEVULINIC ACID SYNTHETASE BY RHODOPSEUDOMONAS SPHEROIDES. Biochim Biophys Acta. 1965 Jan 11;95:94–110. doi: 10.1016/0005-2787(65)90215-7. [DOI] [PubMed] [Google Scholar]
  16. Hadden C., Nester E. W. Purification of competent cells in the Bacillus subtilis transformation system. J Bacteriol. 1968 Mar;95(3):876–885. doi: 10.1128/jb.95.3.876-885.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Hill L. R. An index to deoxyribonucleic acid base compositions of bacterial species. J Gen Microbiol. 1966 Sep;44(3):419–437. doi: 10.1099/00221287-44-3-419. [DOI] [PubMed] [Google Scholar]
  18. Hirota Y. THE EFFECT OF ACRIDINE DYES ON MATING TYPE FACTORS IN ESCHERICHIA COLI. Proc Natl Acad Sci U S A. 1960 Jan;46(1):57–64. doi: 10.1073/pnas.46.1.57. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. IYER V. N., SZYBALSKI W. A MOLECULAR MECHANISM OF MITOMYCIN ACTION: LINKING OF COMPLEMENTARY DNA STRANDS. Proc Natl Acad Sci U S A. 1963 Aug;50:355–362. doi: 10.1073/pnas.50.2.355. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. JOSHI J. G., GUILD W. R., HANDLER P. The presence of two species of DNA in some halobacteria. J Mol Biol. 1963 Jan;6:34–38. doi: 10.1016/s0022-2836(63)80079-0. [DOI] [PubMed] [Google Scholar]
  21. LERMAN L. S. Structural considerations in the interaction of DNA and acridines. J Mol Biol. 1961 Feb;3:18–30. doi: 10.1016/s0022-2836(61)80004-1. [DOI] [PubMed] [Google Scholar]
  22. MARMUR J., DOTY P. Determination of the base composition of deoxyribonucleic acid from its thermal denaturation temperature. J Mol Biol. 1962 Jul;5:109–118. doi: 10.1016/s0022-2836(62)80066-7. [DOI] [PubMed] [Google Scholar]
  23. MARMUR J., DOTY P. Heterogeneity in deoxyribonucleic acids. I. Dependence on composition of the configurational stability of deoxyribonucleic acids. Nature. 1959 May 23;183(4673):1427–1429. doi: 10.1038/1831427a0. [DOI] [PubMed] [Google Scholar]
  24. MARMUR J., FALKOW S., MANDEL M. NEW APPROACHES TO BACTERIAL TAXONOMY. Annu Rev Microbiol. 1963;17:329–372. doi: 10.1146/annurev.mi.17.100163.001553. [DOI] [PubMed] [Google Scholar]
  25. MARTINEZSEGOVIA Z. M., SOKOL F., GRAVES I. L., ACKERMANN W. W. SOME PROPERTIES OF NUCLEIC ACIDS EXTRACTED WITH PHENOL. Biochim Biophys Acta. 1965 Feb 8;95:329–340. doi: 10.1016/0005-2787(65)90497-1. [DOI] [PubMed] [Google Scholar]
  26. MITSUHASHI S., HARADA K., KAMEDA M. Elimination of transmissible drug-resistance by treatment with acriflavin. Nature. 1961 Mar 18;189:947–947. doi: 10.1038/189947a0. [DOI] [PubMed] [Google Scholar]
  27. MacDonald R. E., Turnock G., Forchhammer J. The synthesis and function of ribosomes in a new mutant of Escherichia coli. Proc Natl Acad Sci U S A. 1967 Jan;57(1):141–147. doi: 10.1073/pnas.57.1.141. [DOI] [PMC free article] [PubMed] [Google Scholar]
  28. Moore R. L., McCarthy B. J. Base sequence homology and renaturation studies of the deoxyribonucleic acid of extremely halophilic bacteria. J Bacteriol. 1969 Jul;99(1):255–262. doi: 10.1128/jb.99.1.255-262.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
  29. Moore R. L., McCarthy B. J. Comparative study of ribosomal ribonucleic acid cistrons in enterobacteria and myxobacteria. J Bacteriol. 1967 Oct;94(4):1066–1074. doi: 10.1128/jb.94.4.1066-1074.1967. [DOI] [PMC free article] [PubMed] [Google Scholar]
  30. RITOSSA F. M., SPIEGELMAN S. LOCALIZATION OF DNA COMPLEMENTARY TO RIBOSOMAL RNA IN THE NUCLEOLUS ORGANIZER REGION OF DROSOPHILA MELANOGASTER. Proc Natl Acad Sci U S A. 1965 Apr;53:737–745. doi: 10.1073/pnas.53.4.737. [DOI] [PMC free article] [PubMed] [Google Scholar]
  31. Raymond J. C., Sistrom W. R. The isolation and preliminary characterization of a halophilic photosynthetic bacterium. Arch Mikrobiol. 1967;59(1):255–268. doi: 10.1007/BF00406339. [DOI] [PubMed] [Google Scholar]
  32. SCHILDKRAUT C. L., MARMUR J., DOTY P. Determination of the base composition of deoxyribonucleic acid from its buoyant density in CsCl. J Mol Biol. 1962 Jun;4:430–443. doi: 10.1016/s0022-2836(62)80100-4. [DOI] [PubMed] [Google Scholar]
  33. SHIBA S., TERAWAKI A., TAGUCHI T., KAWAMATA J. Selective inhibition of formation of deoxyribonucleic acid in Escherichia coli by mitomycin C. Nature. 1959 Apr 11;183(4667):1056–1057. doi: 10.1038/1831056a0. [DOI] [PubMed] [Google Scholar]
  34. SUEOKA N., MARMUR J., DOTY P., 2nd Dependence of the density of deoxyribonucleic acids on guanine-cytosine content. Nature. 1959 May 23;183(4673):1429–1431. doi: 10.1038/1831429a0. [DOI] [PubMed] [Google Scholar]
  35. Schumaker V. N., Wagnild J. Zone centrifugation in a cesium chloride density gradient caused by temperature change. Biophys J. 1965 Nov;5(6):947–964. doi: 10.1016/S0006-3495(65)86761-3. [DOI] [PMC free article] [PubMed] [Google Scholar]
  36. Stoeckenius W., Rowen R. A morphological study of Halobacterium halobium and its lysis in media of low salt concentration. J Cell Biol. 1967 Jul;34(1):365–393. doi: 10.1083/jcb.34.1.365. [DOI] [PMC free article] [PubMed] [Google Scholar]
  37. Suyama Y., Gibson J. Satellite DNA in photosynthetic bacteria. Biochem Biophys Res Commun. 1966 Aug 23;24(4):549–553. doi: 10.1016/0006-291x(66)90355-x. [DOI] [PubMed] [Google Scholar]

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