Abstract
Escherichia coli PB160, which carries a tandem duplication with the gene order metB+argH−su159+thi+: metB+argH+su159−thi+, was used to study the mechanism of P1 transduction of genes in the duplicated region. Transduction of the su159+ allele contained within the duplicated segment yields two kinds of su159+ recombinants: 91% are haploid su159+ and 9% are su159+/su159− merodiploids. The duplication in these merodiploid transductants includes the metB locus; however, both copies of the metB locus usually are derived from the recipient. Thus, the requirements for transduction of the “condition of merodiploidy” appear to be the cotransduction of the repeat point (the region where the duplication begins to repeat itself) and, of course, the selected marker (in this case su159+). A mechanism whereby two recipient chromosomes interact with the transduced “repeat point” region to regenerate the tandem duplication is implicated. It appears that a duplication much larger than the quantity of genetic material carried by a P1 phage can be produced in a transductant.
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Selected References
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- CAMPBELL A. Segregants from lysogenic heterogenotes carrying recombinant lambda prophages. Virology. 1963 Jun;20:344–356. doi: 10.1016/0042-6822(63)90124-7. [DOI] [PubMed] [Google Scholar]
- Campbell A. The steric effect in lysogenization by bacteriophage lambda. I. Lysogenization of a partially diploid strain of Escherichia coli K-12. Virology. 1965 Nov;27(3):329–339. doi: 10.1016/0042-6822(65)90112-1. [DOI] [PubMed] [Google Scholar]
- Hill C. W., Foulds J., Soll L., Berg P. Instability of a missense suppressor resulting from a duplication of genetic material. J Mol Biol. 1969 Feb 14;39(3):563–581. doi: 10.1016/0022-2836(69)90146-6. [DOI] [PubMed] [Google Scholar]
- Reid P., Berg P. T4 bacteriophage mutants suppressible by a missense suppressor which inserts glycine in place of arginine for the codon AGA. J Virol. 1968 Sep;2(9):905–914. doi: 10.1128/jvi.2.9.905-914.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Taylor A. L., Trotter C. D. Revised linkage map of Escherichia coli. Bacteriol Rev. 1967 Dec;31(4):332–353. doi: 10.1128/br.31.4.332-353.1967. [DOI] [PMC free article] [PubMed] [Google Scholar]