Skip to main content
NCBI home page
Journal of Bacteriology logoLink to Journal of Bacteriology
. 1969 Aug;99(2):361–365. doi: 10.1128/jb.99.2.361-365.1969

Antifungal Effects of Peroxidase Systems

R I Lehrer 1
PMCID: PMC250023  PMID: 5817553

Abstract

In the presence of hydrogen peroxide and either potassium iodide, sodium chloride, or potassium bromide, purified human myeloperoxidase was rapidly lethal to several species of Candida. Its candidacidal activity was inhibited by cyanide, fluoride, and azide, and by heat inactivation of the enzyme. A hydrogen peroxidegenerating system consisting of d-amino acid oxidase, flavine-adenine dinucleotide, and d-alanine could replace hydrogen peroxide in the candidacidal system. Horseradish peroxidase and human eosinophil granules also exerted candidacidal activity in the presence of iodide and hydrogen peroxide; however, unlike myeloperoxidase or neutrophil granules, these peroxidase sources were inactive when chloride replaced iodide. Cells of Saccharomyces, Geotrichum, and Rhodotorula species, and spores of Aspergillus fumigatus and A. niger were also killed by the combination of myeloperoxidase, iodide, and hydrogen peroxide. Peroxidases, functionally linked to hydrogen peroxide-generating systems, could provide phagocytic cells with the ability to kill many fungal species.

Full text

PDF
361

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. AGNER K. Studies on peroxidative detoxification of purified diphtheria toxin. J Exp Med. 1950 Oct 1;92(4):337–347. doi: 10.1084/jem.92.4.337. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. ARCHER G. T., AIR G., JACKAS M., MORELL D. B. STUDIES ON RAT EOSINOPHIL PEROXIDASE. Biochim Biophys Acta. 1965 Apr 26;99:96–101. doi: 10.1016/s0926-6593(65)80010-8. [DOI] [PubMed] [Google Scholar]
  3. ARCHER G. T., HIRSCH J. G. ISOLATION OF GRANULES FROM EOSINOPHIL LEUCOCYTES AND STUDY OF THEIR ENZYME CONTENT. J Exp Med. 1963 Aug 1;118:277–286. doi: 10.1084/jem.118.2.277. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. AUSTIN L. B., ZELDOW B. J. Quantitative changes of a human salivary bactericidin for Lactobacilli associated with age. Proc Soc Exp Biol Med. 1961 Jun;107:406–408. doi: 10.3181/00379727-107-26641. [DOI] [PubMed] [Google Scholar]
  5. Baehner R. L., Karnovsky M. J., Karnovsky M. L. Degranulation of leukocytes in chronic granulomatous disease. J Clin Invest. 1969 Jan;48(1):187–192. doi: 10.1172/JCI105967. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. FALLON H. J., FREI E., 3rd, DAVIDSON J. D., TRIER J. S., BURK D. Leukocyte preparations from human blood: evaluation of their morphologic and metabolic state. J Lab Clin Med. 1962 May;59:779–791. [PubMed] [Google Scholar]
  7. Holmes B., Page A. R., Good R. A. Studies of the metabolic activity of leukocytes from patients with a genetic abnormality of phagocytic function. J Clin Invest. 1967 Sep;46(9):1422–1432. doi: 10.1172/JCI105634. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. JAGO G. R., MORRISON M. Anti-streptococcal activity of lactoperoxidase III. Proc Soc Exp Biol Med. 1962 Dec;111:585–588. doi: 10.3181/00379727-111-27862. [DOI] [PubMed] [Google Scholar]
  9. Klebanoff S. J., Clem W. H., Luebke R. G. The peroxidase-thiocyanate-hydrogen peroxide antimicrobial system. Biochim Biophys Acta. 1966 Mar 28;117(1):63–72. doi: 10.1016/0304-4165(66)90152-8. [DOI] [PubMed] [Google Scholar]
  10. Klebanoff S. J. Iodination of bacteria: a bactericidal mechanism. J Exp Med. 1967 Dec 1;126(6):1063–1078. doi: 10.1084/jem.126.6.1063. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Klebanoff S. J. Myeloperoxidase-halide-hydrogen peroxide antibacterial system. J Bacteriol. 1968 Jun;95(6):2131–2138. doi: 10.1128/jb.95.6.2131-2138.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Lehrer R. I., Cline M. J. Interaction of Candida albicans with human leukocytes and serum. J Bacteriol. 1969 Jun;98(3):996–1004. doi: 10.1128/jb.98.3.996-1004.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. McRipley R. J., Sbarra A. J. Role of the phagocyte in host-parasite interactions. XI. Relationship between stimulated oxidative metabolism and hydrogen peroxide formation, and intracellular killing. J Bacteriol. 1967 Nov;94(5):1417–1424. doi: 10.1128/jb.94.5.1417-1424.1967. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. McRipley R. J., Sbarra A. J. Role of the phagocyte in host-parasite interactions. XII. Hydrogen peroxide-myeloperoxidase bactericidal system in the phagocyte. J Bacteriol. 1967 Nov;94(5):1425–1430. doi: 10.1128/jb.94.5.1425-1430.1967. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. PROCKNOW J. J. Treatment of opportunistic fungus infections. Lab Invest. 1962 Nov;11:1217–1230. [PubMed] [Google Scholar]
  16. SCHULTZ J., KAMINKER K. Myeloperoxidase of the leucocyte of normal human blood. I. Content and localization. Arch Biochem Biophys. 1962 Mar;96:465–467. doi: 10.1016/0003-9861(62)90321-1. [DOI] [PubMed] [Google Scholar]
  17. SCHULTZ J., SHMUKLER H. W. MYELOPEROXIDASE OF THE LEUCOCYTE OF NORMAL HUMAN BLOOD. II. ISOLATION, SPECTROPHOTOMETRY, AND AMINO ACID ANALYSIS. Biochemistry. 1964 Sep;3:1234–1238. doi: 10.1021/bi00897a009. [DOI] [PubMed] [Google Scholar]
  18. Williams H. E. Alpha-glucosidase activity in human leucocytes. Biochim Biophys Acta. 1966 Jul 27;124(1):34–38. doi: 10.1016/0304-4165(66)90310-2. [DOI] [PubMed] [Google Scholar]
  19. ZELDOW B. J. Studies on the antibacterial action of human saliva. III. Cofactor requirements of Lactobacillus bactericidin. J Immunol. 1963 Jan;90:12–16. [PubMed] [Google Scholar]

Articles from Journal of Bacteriology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES