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. 1969 Aug;99(2):418–421. doi: 10.1128/jb.99.2.418-421.1969

Intracellular Protein Breakdown in a Thermophile

Israel Epstein 1, Nathan Grossowicz 1
PMCID: PMC250033  PMID: 4897108

Abstract

Protein breakdown of 5 to 7% per hr was found in nitrogen-starved cells of an unclassified prototrophic thermophilic bacillus; a similar protein-breakdown rate (6.5% per hr) was found in resting cells of Escherichia coli. In the thermophile, the rate of protein breakdown was markedly influenced by the temperature; it was maximal between 45 and 55 C, and it decreased considerably at 35 and 75 C, temperatures which are only slightly below or above the minimal and maximal growth temperatures. Growing cultures of the thermophile showed little, if any, protein breakdown, a finding similar to that of others with E. coli.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. ALLEN M. B. The thermophilic aerobic sporeforming bacteria. Bacteriol Rev. 1953 Jun;17(2):125–173. doi: 10.1128/br.17.2.125-173.1953. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. ANRAKU Y., ANDOH T., MIZUNO D. THE TURNOVER OF RNA AND OF PROTEIN OF E. COLI IN THE SULFUR-DEFICIENT STATE. J Biochem. 1963 Sep;54:246–252. doi: 10.1093/oxfordjournals.jbchem.a127779. [DOI] [PubMed] [Google Scholar]
  3. Borek E., Ponticorvo L., Rittenberg D. PROTEIN TURNOVER IN MICRO-ORGANISMS. Proc Natl Acad Sci U S A. 1958 May;44(5):369–374. doi: 10.1073/pnas.44.5.369. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Bubela B., Holdsworth E. S. Amino acid uptake, protein and nucleic acid synthesis and turnover in Bacillus stearothermophilus. Biochim Biophys Acta. 1966 Aug 17;123(2):364–375. doi: 10.1016/0005-2787(66)90289-9. [DOI] [PubMed] [Google Scholar]
  5. COWIE D. B., BOLTON E. T., SANDS M. K. Sulfur metabolism in Escherichia coli. I. Sulfate metabolism of normal and mutant cells. J Bacteriol. 1950 Sep;60(3):233–248. doi: 10.1128/jb.60.3.233-248.1950. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Epstein I., Grossowicz N. Prototrophic thermophilic bacillus: isolation, properties, and kinetics of growth. J Bacteriol. 1969 Aug;99(2):414–417. doi: 10.1128/jb.99.2.414-417.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. KOCH A. L., LEVY H. R. Protein turnover in growing cultures of Escherichia coli. J Biol Chem. 1955 Dec;217(2):947–957. [PubMed] [Google Scholar]
  8. KOFFLER H., GALE G. O. The relative thermostability of cytoplasmic proteins from thermophilic bacteria. Arch Biochem Biophys. 1957 Mar;67(1):249–251. doi: 10.1016/0003-9861(57)90267-9. [DOI] [PubMed] [Google Scholar]
  9. Kornberg A., Spudich J. A., Nelson D. L., Deutscher M. P. Origin of proteins in sporulation. Annu Rev Biochem. 1968;37:51–78. doi: 10.1146/annurev.bi.37.070168.000411. [DOI] [PubMed] [Google Scholar]
  10. MANDELSTAM J. The intracellular turnover of protein and nucleic acids and its role in biochemical differentiation. Bacteriol Rev. 1960 Sep;24(3):289–308. doi: 10.1128/br.24.3.289-308.1960. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. MANDELSTAM J. Turnover of protein in growing and non-growing populations of Escherichia coli. Biochem J. 1958 May;69(1):110–119. doi: 10.1042/bj0690110. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. MONRO R. E. Protein turnover and the formation of protein inclusions during sporulation of Bacillus thuringiensis. Biochem J. 1961 Nov;81:225–232. doi: 10.1042/bj0810225. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. PROCTOR M. H. Protein turnover in resting bacteria. Folia Microbiol (Praha) 1962 Jul;7:207–215. doi: 10.1007/BF02930765. [DOI] [PubMed] [Google Scholar]
  14. ROTMAN B., SPIEGELMAN S. On the origin of the carbon in the induced synthesis beta-galactosidase in Escherichia coli. J Bacteriol. 1954 Oct;68(4):419–429. doi: 10.1128/jb.68.4.419-429.1954. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. URBA R. C. Protein breakdown in Bacillus cereus. Biochem J. 1959 Mar;71(3):513–518. doi: 10.1042/bj0710513. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Willetts N. S. Intracellular protein breakdown in growing cells of Escherichia coli. Biochem J. 1967 May;103(2):462–466. doi: 10.1042/bj1030462. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Willetts N. S. Protein degradation during diauxic growth of Escerichia coli. Biochem Biophys Res Commun. 1965 Sep 22;20(6):692–696. doi: 10.1016/0006-291x(65)90071-9. [DOI] [PubMed] [Google Scholar]

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