Skip to main content
NCBI home page
Journal of Bacteriology logoLink to Journal of Bacteriology
. 1969 Aug;99(2):527–530. doi: 10.1128/jb.99.2.527-530.1969

Estimation and Identification of Streptococcus dysgalactiae Protective Antibody in Sera of Rabbits and Cattle

D M Stark 1, N L Norcross 1, J E Leahey 1
PMCID: PMC250050  PMID: 4980066

Abstract

Rabbit and cow anti-Streptococcus dysgalactiae sera were tested by bacterial agglutination, complement fixation, hemagglutination, and immunodiffusion for the presence of antibody. The results of these tests were compared with mouse-protection studies on the same serum to estimate which in vitro test would best reflect the in vivo protective capacity of serum. Identification of the antibody constituents responsible for the mouse protection, hemagglutination, and complement fixation titers were established by reacting whole and diluted antisera with mercaptoethanol before and after testing. Results indicate that the complement fixation test may be a more accurate indicator of IgG protective bovine and rabbit antibody, whereas the hemagglutination test may more readily reflect a wider range of protective antibody levels and IgM. The complement fixation test showed some shared responses to IgG and IgM in both the rabbit and cow, whereas the IgM components seemed to be the predominant factor influencing hemagglutination titers in the rabbit and more so in the bovine. Mouse protection tests with mercaptoethanol-treated cow and rabbit sera indicate that the protective capacity of these antisera is shared between IgM and IgG components.

Full text

PDF
527

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. BAKSHI S. N., SINGH C. M. ANTIGENIC RELATIONSHIP BETWEEN MASTITIS STREPTOCOCCI BELONGING TO GROUP-C. I. STUDIES BY AGGLUTINATION, PRECIPITATION, PRECIPITATION ABSORPTION AND PRECIPITATION IN AGAR GEL. J Comp Pathol. 1964 Jul;74:398–408. doi: 10.1016/s0368-1742(64)80045-x. [DOI] [PubMed] [Google Scholar]
  2. CAMPBELL S. G., NORCROSS N. L. ANTIBODIES AGAINST STREPTOCOCCUS AGALACTIAE IN THE COLOSTRUM OF FIRST-CALF HEIFERS. Am J Vet Res. 1964 Jul;25:993–997. [PubMed] [Google Scholar]
  3. DEUTSCH H. F., MORTON J. I. Dissociation of human serum macroglobulins. Science. 1957 Mar 29;125(3248):600–601. doi: 10.1126/science.125.3248.600. [DOI] [PubMed] [Google Scholar]
  4. GRUBB R., SWAHN B. Destruction of some agglutinins but not of others by two sulfhydryl compounds. Acta Pathol Microbiol Scand. 1958;43(3):305–309. doi: 10.1111/j.1699-0463.1958.tb04899.x. [DOI] [PubMed] [Google Scholar]
  5. Goldstein I., Caravano R. Determination of anti group A streptococcal polysaccharide antibodies in human sera by an hemagglutination technique. Proc Soc Exp Biol Med. 1967 Apr;124(4):1209–1212. doi: 10.3181/00379727-124-31967. [DOI] [PubMed] [Google Scholar]
  6. HALBERT S. P., SWICK L., SONN C. The use of precipitin analysis in agar for the study of human streptococcal infections. I. Oudin technic. J Exp Med. 1955 May 1;101(5):539–556. doi: 10.1084/jem.101.5.539. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. HOLMAN H. H., SMITH I. M., PATTISON I. H. Experimental mastitis produced in goats by Streptococcus dysgalactiae. J Comp Pathol. 1953 Jul;63(3):199–210. doi: 10.1016/s0368-1742(53)80024-x. [DOI] [PubMed] [Google Scholar]
  8. Lancefield R. C., Freimer E. H. Type-specific polysaccharide antigens of group B streptococci. J Hyg (Lond) 1966 Jun;64(2):191–203. doi: 10.1017/s0022172400040456. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. NETER E. Bacterial hemagglutination and hemolysis. Bacteriol Rev. 1956 Sep;20(3):166–188. doi: 10.1128/br.20.3.166-188.1956. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. NORCROSS N. L. Antigenic substances purified from Streptococcus agalactiae. I. Antibody response in infected cattle. Cornell Vet. 1963 Apr;53:301–308. [PubMed] [Google Scholar]
  11. Norcross N. L., Dodd K., Stark D. M. Use of the mouse protection test to assay streptococci antibodies in bovine serum and milk. Am J Vet Res. 1968 Jun;29(6):1201–1205. [PubMed] [Google Scholar]
  12. Pike R. M. Antibody heterogeneity and serological reactions. Bacteriol Rev. 1967 Jun;31(2):157–174. doi: 10.1128/br.31.2.157-174.1967. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Stark D. M., Norcross N. L. A passive hemagglutination technique for detection of Streptococcus dysgalactiae antibody. Am J Vet Res. 1969 Jan;30(1):127–132. [PubMed] [Google Scholar]
  14. UHR J. W., FINKELSTEIN S., FRANKLIN E. C. Antibody response to bacteriophage phi-X-174 in non-mammalian vertebrates. Proc Soc Exp Biol Med. 1962 Oct;111:13–15. doi: 10.3181/00379727-111-27691. [DOI] [PubMed] [Google Scholar]

Articles from Journal of Bacteriology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES