Abstract
Cell-free extracts of Escherichia coli were active in catalyzing the synthesis of a biotin vitamer from 7,8-diaminopelargonic acid. The vitamer was identified as desthiobiotin on the basis of its chromatographic and electrophoretic characteristics and its biotin activities for a variety of microorganisms. The reaction was stimulated five-fold by bicarbonate, suggesting that an “active CO2” was incorporated into the carbonyl carbon of desthiobiotin. The enzyme was demonstrable in a wild-type (K-12) and in all biotin mutants of E. coli that were tested, with the exception of a strain which was able to grow on desthiobiotin but not on diaminopelargonic acid. Furthermore, the enzyme was repressible by biotin in all of the strains tested. These results are consistent with the hypothesis that the biosynthesis of desthiobiotin from 7,8-diaminopelargonic acid is an obligatory step in the biosynthetic pathway of biotin in E. coli.
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- DAVIS B. D., MINGIOLI E. S. Mutants of Escherichia coli requiring methionine or vitamin B12. J Bacteriol. 1950 Jul;60(1):17–28. doi: 10.1128/jb.60.1.17-28.1950. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Del Campillo-Campbell A., Kayajanian G., Campbell A., Adhya S. Biotin-requiring mutants of Escherichia coli K-12. J Bacteriol. 1967 Dec;94(6):2065–2066. doi: 10.1128/jb.94.6.2065-2066.1967. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Eisenberg M. A., Star C. Synthesis of 7-oxo-8-aminopelargonic acid, a biotin vitamer, in cell-free extracts of Escherichia coli biotin auxotrophs. J Bacteriol. 1968 Oct;96(4):1291–1297. doi: 10.1128/jb.96.4.1291-1297.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
- LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
- PAI C. H., LICHSTEIN H. C. THE BIOSYNTHESIS OF BIOTIN IN MICROORGANISMS. II. MECHANISM OF THE REGULATION OF BIOTIN SYNTHESIS IN ESCHERICHIA COLI. Biochim Biophys Acta. 1965 Apr 12;100:36–42. doi: 10.1016/0304-4165(65)90424-1. [DOI] [PubMed] [Google Scholar]
- Pai C. H., Lichstein H. C. Biosynthesis of biotin in microorganisms. IV. Repression and derepression of (+ -)-biotin synthesis from (+)-desthiobiotin. Arch Biochem Biophys. 1966 Apr;114(1):138–144. doi: 10.1016/0003-9861(66)90314-6. [DOI] [PubMed] [Google Scholar]
- Pai C. H., Lichstein H. C. Biosynthesis of biotin in microorganisms. VI. Further evidence for desthiobiotin as a precursor in Eschericia coli. J Bacteriol. 1967 Dec;94(6):1930–1933. doi: 10.1128/jb.94.6.1930-1933.1967. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Rolfe B., Eisenberg M. A. Genetic and biochemical analysis of the biotin loci of Escherichia coli K-12. J Bacteriol. 1968 Aug;96(2):515–524. doi: 10.1128/jb.96.2.515-524.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]