Abstract
Most of the genetic studies on streptomycetes have been done with cultures erroneously designated as Streptomyces coelicolor. To determine whether these cultures are genetically homologous with the S. violaceoruber nominifer, their deoxyribonucleic acids (DNA) were analyzed, and selected pairs of mutants were crossed. The four cultures used in genetic studies, and called S. coelicolor in the literature, were found to constitute a genospecies, based upon DNA hybridization and recombination tests. In addition, DNA from Actinopycnidium caeruleum formed extensive duplexes with S. violaceoruber DNA. S. violaceoruber cultures and A. caeruleum were distinctly different from the S. coelicolor nominifer.
Full text
PDF




Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- BRADLEY S. G. Heterokaryosis in Streptomyces coelicolor. J Bacteriol. 1957 Apr;73(4):581–582. doi: 10.1128/jb.73.4.581-582.1957. [DOI] [PMC free article] [PubMed] [Google Scholar]
- BRAENDLE D. H., SZYBALSKI W. Heterokaryotic compatibility, metabolic cooperation, and genic recombination in Streptomyces. Ann N Y Acad Sci. 1959 Sep 30;81:824–853. doi: 10.1111/j.1749-6632.1959.tb49369.x. [DOI] [PubMed] [Google Scholar]
- HOPWOOD D. A. Linkage and the mechanism of recombination in Streptomyces coelicolor. Ann N Y Acad Sci. 1959 Sep 30;81:887–898. doi: 10.1111/j.1749-6632.1959.tb49374.x. [DOI] [PubMed] [Google Scholar]
- Huang P. C., Rosenberg E. Determination of DNA base composition via depurination. Anal Biochem. 1966 Jul;16(1):107–113. doi: 10.1016/0003-2697(66)90085-6. [DOI] [PubMed] [Google Scholar]
- KUTZNER H. J., WAKSMAN S. A. Streptomyces coelicolor Mueller and Streptomyces violaceoruber Waksman and Curtis, two distinctly different organisms. J Bacteriol. 1959 Oct;78:528–538. doi: 10.1128/jb.78.4.528-538.1959. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Meselson M., Stahl F. W., Vinograd J. EQUILIBRIUM SEDIMENTATION OF MACROMOLECULES IN DENSITY GRADIENTS. Proc Natl Acad Sci U S A. 1957 Jul 15;43(7):581–588. doi: 10.1073/pnas.43.7.581. [DOI] [PMC free article] [PubMed] [Google Scholar]
- SERMONTI G., SPADA-SERMONTI I. Gene recombination in Streptomyces coelicolor. J Gen Microbiol. 1956 Dec;15(3):609–616. doi: 10.1099/00221287-15-3-609. [DOI] [PubMed] [Google Scholar]
- Tewfik E. M., Bradley S. G. Characterization of deoxyribonucleic acids from streptomycetes and nocardiae. J Bacteriol. 1967 Dec;94(6):1994–2000. doi: 10.1128/jb.94.6.1994-2000.1967. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Warmaar S. O., Cohen J. A. A quantitative assay for DNA-DNA hybrids using membrane filters. Biochem Biophys Res Commun. 1966 Aug 23;24(4):554–558. doi: 10.1016/0006-291x(66)90356-1. [DOI] [PubMed] [Google Scholar]
- YAMAGUCHI T. COMPARISON OF THE CELL-WALL COMPOSITION OF MORPHOLOGICALLY DISTINCT ACTINOMYCETES. J Bacteriol. 1965 Feb;89:444–453. doi: 10.1128/jb.89.2.444-453.1965. [DOI] [PMC free article] [PubMed] [Google Scholar]