Abstract
Nonconidiating (Con−) mutants were isolated from wild-type and color mutants of the fungus Trichoderma viride Pers. ex Fries. Heterokaryons were easily produced and maintained, and the complementation relationships among the Con− mutants were established. Most Con− mutants could complement one or more of the other Con− mutants. When marked Con− mutants were mixed with marked Con+ testers, conidiating heterokaryons were formed. The conidia thus obtained produced only the parental type colonies after replating, indicating that nonconidiation is a nuclear characteristic. Allowing two Con− colonies to meet and produce a heterokaryon, it was found that the migration of nuclei reached a rate of 5 mm per hr, which is several times greater than the rate of hyphal elongation; it was also found that heterokaryosis of a mycelial region preceded its ability to conidiate.
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