Abstract
In Staphylococcus aureus, vitamin K2 isoprenologues can be labeled with 14C-acetate, mevalonate, glycerol, α-naphthol, and menadione. All the radioactivity from α-naphthol is in the ring nucleus. Eighty-six per cent of the radioactivity from mevalonate is in the side chain. The labeled isoprenologues of vitamin K2 can be separated and recovered quantitatively. The rates of incorporation and turnover after pulse-chase experiments indicate that the isoprenologues do not lose radioactivity once they are formed. The kinetics of incorporation of radioactivity into the ring nucleus of each isoprenologue are the same. The alkylation of the ring does not involve a simple sequential addition of isoprenoid units of the same specific activity. The incorporation of mevalonate shows that shorter side chain isoprenologues have a higher specific activity per five carbon unit than the longer side chain isoprenologues. Perhaps the alkyl precursors are from different pools since the kinetics of incorporation into all isoprenologues are different.
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