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. 1969 Nov;100(2):687–694. doi: 10.1128/jb.100.2.687-694.1969

Influence of Methionine Pool Composition on the Formation of Methyl-Deficient Transfer Ribonucleic Acid in Saccharomyces cerevisiae

Kerstin Kjellin-Stråby a,1
PMCID: PMC250145  PMID: 5354940

Abstract

Methionine auxotrophs of Saccharomyces cerevisiae continue to synthesize ribonucleic acid (RNA) after methionine withdrawal. The newly synthesized transfer RNA (tRNA) is methyl-deficient in some strains, but not in all. Whether such tRNA will accumulate depends on the position of the block in the methionine pathway that is carried by the mutant strain. Free methionine rapidly decreases in the intracellular pool of all strains after its removal from the medium. Certain metabolites derived from methionine are removed from the pool relatively slowly after methionine withdrawal. Notable among these is S-adenosylhomocysteine, which is depleted less rapidly from those strains that accumulate methyl-deficient tRNA than from others. S-adenosylhomocysteine is a potent inhibitor of tRNA-methylating enzymes in vitro.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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